Confirmatory analysis of buprenorphine, norbuprenorphine, and glucuronide metabolites in plasma by LCMSMS. Application to umbilical cord plasma from buprenorphine-maintained pregnant women
| Autor: | Rolley E. Johnson, Marilyn A. Huestis, Hendrée E. Jones, Marta Concheiro, Diaa M. Shakleya |
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| Rok vydání: | 2010 |
| Předmět: |
Buprenorphine+Norbuprenorphine
Narcotic Antagonists Metabolite Clinical Biochemistry Ion suppression in liquid chromatography–mass spectrometry Sensitivity and Specificity Biochemistry Article Analytical Chemistry chemistry.chemical_compound Glucuronides Pregnancy Tandem Mass Spectrometry medicine Humans Norbuprenorphine Umbilical cord plasma Chromatography Reproducibility of Results Cell Biology General Medicine Fetal Blood Opioid-Related Disorders Buprenorphine chemistry Neonatal outcomes Linear Models Female Glucuronide Chromatography Liquid medicine.drug |
| Zdroj: | Journal of Chromatography B. 878:13-20 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2009.11.005 |
| Popis: | An LCMSMS method was developed and fully validated for the simultaneous quantification of buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine-glucuronide (BUP-Gluc), and norbuprenorphine-glucuronide (NBUP-Gluc) in 0.5mL plasma, fulfilling confirmation criteria with two transitions for each compound with acceptable relative ion intensities. Transitions monitored were 468.3>396.2 and 468.3>414.3 for BUP, 414.3>340.1 and 414.3>326.0 for NBUP, 644.3>468.1 and 644.3>396.3 for BUP-Gluc, and 590.3>414.3 and 590.3>396.2 for NBUP-Gluc. Linearity was 0.1-50ng/mL for BUP and BUP-Gluc, and 0.5-50ng/mL for NBUP and NBUP-Gluc. Intra-day, inter-day, and total assay imprecision (%RSD) were |
| Databáze: | OpenAIRE |
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