Different parkinsonism models produce a time-dependent induction of COX-2 in the substantia nigra of rats
| Autor: | Sergio Tufik, M.M.S. Lima, Angela B. Reksidler, Hidevaldo B. Machado, Silvio M. Zanata, Maria A.B.F. Vital |
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| Rok vydání: | 2006 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Time Factors Parkinson's disease Tyrosine 3-Monooxygenase Blotting Western Central nervous system Gene Expression Substantia nigra Striatum Biology chemistry.chemical_compound Parkinsonian Disorders Internal medicine Reaction Time medicine Animals Neurotoxin Rats Wistar Oxidopamine Molecular Biology Analysis of Variance Behavior Animal General Neuroscience MPTP Parkinsonism MPTP Poisoning Immobility Response Tonic medicine.disease Rats Substantia Nigra Disease Models Animal medicine.anatomical_structure Endocrinology nervous system chemistry Cyclooxygenase 2 Anesthesia Cyclooxygenase 1 Neurology (clinical) Locomotion Developmental Biology |
| Zdroj: | Brain Research. 1101:117-125 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2006.05.016 |
| Popis: | The present study investigated the effects on general activity, COX-2 and TH protein expression of intranigral neurotoxins LPS, MPTP or 6-OHDA infusion in rats. Results indicate that LPS produced an increase in locomotion frequency (3 and 7 days after surgery) and a strong up-regulation of COX-2 protein 16 and 24 h after surgery, as observed in the substantia nigra (SN). The MPTP model generated impairment in locomotion frequency 24 h after surgery. Besides, MPTP caused a marked up-regulation in COX-2 protein observed in the SN 16 h after surgery. Moreover, the 6-OHDA model produced severe motor impairment indicated by the decrease in locomotion (24 h) and rearing (24 h, 3 and 7 days) frequencies and also an increase in latency (24 h, 3 and 7 days) and immobility (24 h and 3 days) times. We also demonstrated an up-regulation of COX-2, which occurred in the SN 4-24 h after surgery. TH protein did not appear to be reduced in the striatum in the groups lesioned with the neurotoxins. In contrast, the TH content of SN was significantly reduced in the groups lesioned with the very same neurotoxins. For all the models analyzed, we observed no statistical differences in the expression of COX-2 in the striatum along the time-points. The results of the present study suggest that COX-2 induction patterns differ in function of the neurotoxin tested. Such time-dependent induction has been found to be relatively constant, a fact of great significance considering the importance of the neuroinflammatory process in Parkinson's disease. |
| Databáze: | OpenAIRE |
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