Population genetics of Leishmania (Leishmania) major DNA isolated from cutaneous leishmaniasis patients in Pakistan based on multilocus microsatellite typing
| Autor: | Yoshihisa Hashiguchi, Mohammad Zahangir Alam, Gabriele Schönian, Hirotomo Kato, Ken Katakura, Javed Hussain Baloch, Ryo Nakao, Abdul Manan Bhutto, Hiroshi Uezato, Farooq Rahman Soomro |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Population genetics
Population Leishmaniasis Cutaneous Cutaneous leishmaniasis Microsatellite typing medicine Humans Leishmania major Pakistan Genetic variability education Phylogeny Genetics education.field_of_study Polymorphism Genetic biology Research Leishmania (Leishmania) major DNA Protozoan medicine.disease biology.organism_classification Infectious Diseases Genetic marker Multilocus sequence typing Microsatellite Parasitology Microsatellite Repeats Multilocus Sequence Typing |
| Zdroj: | Parasites & Vectors |
| ISSN: | 1756-3305 |
| Popis: | Background: Cutaneous leishmaniasis (CL) is a major and fast increasing public health problem, both among the local Pakistani populations and the Afghan refugees in camps. Leishmania (Leishmania) major is one of the etiological agents responsible for CL in Pakistan. Genetic variability and population structure have been investigated for 66 DNA samples of L. (L.) major isolated from skin biopsy of CL patients. Methods: Multilocus microsatellite typing (MLMT), employing 10 independent genetic markers specific to L. (L.) major, was used to investigate the genetic polymorphisms and population structures of Pakistani L. (L.) major DNA isolated from CL human cases. Their microsatellite profiles were compared to those of 130 previously typed strains of L. (L.) major from various geographical localities. Results: All the markers were polymorphic and fifty-one MLMT profiles were recognized among the 66 L. (L.) major DNA samples. The data displayed significant microsatellite polymorphisms with rare allelic heterozygosities. A Bayesian model-based approach and phylogenetic analysis inferred two L. (L.) major populations in Pakistan. Thirty-four samples belonged to one population and the remaining 32 L. (L.) major samples grouped together into another population. The two Pakistani L. (L.) major populations formed separate clusters, which differ genetically from the populations of L. (L.) major from Central Asia, Iran, Middle East and Africa. Conclusions: The considerable genetic variability of L. (L.) major might be related to the existence of different species of sand fly and/or rodent reservoir host in Sindh province, Pakistan. A comprehensive study of the epidemiology of CL including the situation or spreading of reservoirs and sand fly vectors in these foci is, therefore, warranted. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink