The Effects of Different Intraabdominal Pressure Protocols in Laparoscopic Procedures on Oxidative Stress Markers and Morphology in Rat Ovaries

Autor: Levent Akman, Ebru Sezer, Ozlem Yilmaz-Dilsiz, Alper Biler, Yiğit Uyanıkgil, Utku Ateş, Sait Yücebilgin, Fatih Sendag, Ali Akdemir
Přispěvatelé: Ege Üniversitesi
Rok vydání: 2014
Předmět:
Insufflation
Laparoscopic surgery
medicine.medical_specialty
Time Factors
medicine.medical_treatment
Rat model
laparoscopy
Urology
Medicine (miscellaneous)
Abdominal cavity
medicine.disease_cause
General Biochemistry
Genetics and Molecular Biology
Rats
Sprague-Dawley
Pneumoperitoneum
Malondialdehyde
Pressure
Internal Medicine
medicine
Animals
oxidative stress
Pharmacology (medical)
Genetics (clinical)
Carbon dioxide insufflation
business.industry
Ovary
ComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKS
Abdominal Cavity
Carbon Dioxide
medicine.disease
reperfusion
Surgery
body regions
ComputingMilieux_MANAGEMENTOFCOMPUTINGANDINFORMATIONSYSTEMS
ComputingMethodologies_PATTERNRECOGNITION
medicine.anatomical_structure
ischemic preconditioning
Reperfusion Injury
Reviews and References (medical)
Intraabdominal pressure
Female
pneumoperitoneum
InformationSystems_MISCELLANEOUS
business
Pneumoperitoneum
Artificial
Biomarkers
Oxidative stress
Zdroj: ResearcherID
Scopus-Elsevier
ISSN: 1899-5276
2561-8113
DOI: 10.17219/acem/37331
Popis: WOS: 000351333600005
PubMed ID: 25618113
Background. To determine the effects of different intraabdominal pressure (TAP) on the ovaries in a laparoscopic rat model. Objectives. The aim of the study was to determine the effects on the ovaries of different intraabdominal pressures (TAP) in laparoscopic surgery in a rat model. Material and Methods. Thirty-two post-pubertal nonpregnant Sprague-Dawley rats were divided randomly into four groups. In the control group, no intraabdominal pressure (TAP) was applied. In Group Pp10 and Group Pp15, an TAP of 10 and 15 mm Hg, respectively, were applied by carbon dioxide insufflation for 60 min, and a 30-min desufflation was carried out. In Group IPp15, a 15 mm Hg TAP was applied for 10 min, and then CO2 was desufflated for 10 min. After this ischemic preconditioning, IAP was established at 15 mm Hg for 60 min, after which CO2 was desufflated for 30 min. Erythrocyte and ovarian tissue maiondialdehyde (MDA) and histopathologic damage scores were evaluated. Results. In Groups Pp10 and Pp 15, ovarian tissue MDA values were significantly increased compared to the control group. In Groups Pp10 and Pp15, erythrocyte MDA values were significantly increased when compared to Group IPp15 and the control group. Ovarian histopatological assesment scores were significantly higher in Group Pp15 than in Groups Ppl 0 and IPp15. Conclusions. Pneumoperitoneum causes injuries to abdominal organ such as the ovaries. The ischemic preconditioning method is more effective in reducing oxidative stress due to laparoscopic pneumoperitoneum than lowpressure pneumoperitoneum methods
Databáze: OpenAIRE
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