Gene-specific Transcriptional Activation Mediated by the p150 Subunit of the Chromatin Assembly Factor 1
| Autor: | Derick S. C. Ou, Chung-Fan Lee, Li-Jung Juan, Sung Bau Lee |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Gene Expression Regulation
Viral Transcriptional Activation Nucleosome assembly Chromosomal Proteins Non-Histone Cytomegalovirus P300-CBP Transcription Factors Transfection Biochemistry DNA-binding protein Immediate-Early Proteins Cell Line Tumor Humans Transcription Chromatin and Epigenetics p300-CBP Transcription Factors Chromatin Assembly Factor-1 Promoter Regions Genetic Base Pairing Molecular Biology biology Chromatin Assembly Factor I Promoter Cell Biology Molecular biology Protein Structure Tertiary DNA-Binding Proteins Protein Subunits Histone biology.protein Chromatin immunoprecipitation Protein Binding Transcription Factors |
| Zdroj: | Journal of Biological Chemistry. 284:14040-14049 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m901833200 |
| Popis: | Chromatin assembly factor 1 contains three subunits, p150, p60, and p48. It is essential for coupling nucleosome assembly to newly synthesized DNA. Whether chromatin assembly factor 1 subunits have functions beyond escorting histones, which depends on the complex formation of p150 and p60, has been an issue of great interest. This study reveals a novel role of p150, but not p60, in gene-specific transcriptional activation. We found that p150 transcriptionally activated an essential viral promoter, the major immediate early promoter (MIEP) of the human cytomegalovirus, independently of p60. Knocking down p150 decreased the MIEP function in both transfected and virally infected cells. The chromatin immunoprecipitation analysis and the in vitro protein-DNA binding assay demonstrated that p150 used its KER domain to associate with the MIEP from –593 to –574 bp. The N-terminal 244 residues were also found essential for p150-mediated MIEP activation, likely through recruiting the acetyltransferase p300 to acetylate local histones. Domain swapping experiments further showed that the KER and the N terminus of p150 acted as an independent DNA binding and transcriptional activation domain, respectively. Because p60 did not seem involved in the reaction, together these results indicate for the first time that p150 directly activates transcription, independently of its histone deposition function. |
| Databáze: | OpenAIRE |
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