Identification and characterization of the human MOG1 gene
| Autor: | Kavita A. Marfatia, Michelle T. Harreman, Anita H. Corbett, Paula M. Vertino, Patrizia Fanara |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Recombinant Fusion Proteins Green Fluorescent Proteins Molecular Sequence Data Saccharomyces cerevisiae Gene Expression Plasma protein binding Biology Cell Line Two-Hybrid System Techniques Tumor Cells Cultured Genetics Humans Tissue Distribution Amino Acid Sequence RNA Messenger Nuclear protein Peptide sequence Gene Cell Nucleus Sequence Homology Amino Acid Genetic Complementation Test Nuclear Proteins General Medicine Blotting Northern biology.organism_classification Luminescent Proteins ran GTP-Binding Protein TAF4 Mutation Ran Female Nuclear transport Protein Binding |
| Zdroj: | Gene. 266:45-56 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(01)00364-x |
| Popis: | Many of the proteins that mediate transport into and out of the nucleus have been structurally and functionally conserved throughout evolution. Here we describe the sequence and characterization of the human MOG1 gene. The MOG1 gene was originally identified in Saccharomyces cerevisiae as a multi-copy suppressor of conditional alleles of the yeast nuclear transport factor, GSP1 (scRan) (Oki and Nishimoto (1998) Proc. Natl. Acad. Sci. USA 95, 15388-15393). A search of the expressed sequence tag database identified a putative human protein that is 29% identical and 47% similar to the yeast protein. Our experiments demonstrate that the human MOG1 message is expressed in a variety of tissue samples. Several experiments indicate that the human MOG1 protein binds to both yeast and human Ran suggesting functional conservation between the yeast and human MOG1 proteins. Furthermore, hMOG1a, like scMOG1, is localized throughout the cell but is concentrated within the nucleus. Consistent with these findings, hMOG1a can partially complement the growth defect present in yeast MOG1 deletion cells. Taken together, our findings suggest that MOG1 is an evolutionarily conserved Ran binding protein that could play a role in regulating nuclear protein trafficking. |
| Databáze: | OpenAIRE |
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