Early mouse caudal development relies on crosstalk between retinoic acid, Shh and Fgf signalling pathways
| Autor: | Muriel Rhinn, Vanessa Ribes, Isabelle Le Roux, Brigitte Schuhbaur, Pascal Dollé |
|---|---|
| Přispěvatelé: | Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, Peney, Maité, Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
animal structures
Primitive Streak Neurogenesis Retinoic acid Kruppel-Like Transcription Factors Tretinoin Biology Zinc Finger Protein Gli2 Fibroblast growth factor Models Biological Zinc Finger Protein GLI1 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Animals Hedgehog Proteins [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology 030304 developmental biology Body Patterning Genetics 0303 health sciences Neural Plate Primitive streak Embryogenesis Gastrulation Gene Expression Regulation Developmental Embryo Mammalian Aldehyde Oxidoreductases Cell biology Neuroepithelial cell Fibroblast Growth Factors chemistry Spinal Cord Epiblast embryonic structures 030217 neurology & neurosurgery Biomarkers Developmental Biology Signal Transduction |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 2009, 136 (4), pp.665-76. ⟨10.1242/dev.016204⟩ Development (Cambridge, England), 2009, 136 (4), pp.665-76. ⟨10.1242/dev.016204⟩ |
| ISSN: | 1477-9129 0950-1991 |
| Popis: | International audience; The progressive generation of embryonic trunk structures relies on the proper patterning of the caudal epiblast, which involves the integration of several signalling pathways. We have investigated the function of retinoic acid (RA) signalling during this process. We show that, in addition to posterior mesendoderm, primitive streak and node cells transiently express the RA-synthesizing enzyme Raldh2 prior to the headfold stage. RA-responsive cells (detected by the RA-activated RARE-lacZ transgene) are additionally found in the epiblast layer. Analysis of RA-deficient Raldh2(-/-) mutants reveals early caudal patterning defects, with an expansion of primitive streak and mesodermal markers at the expense of markers of the prospective neuroepithelium. As a result, many genes involved in neurogenesis and/or patterning of the embryonic spinal cord are affected in their expression. We demonstrate that RA signalling is required at late gastrulation stages for mesodermal and neural progenitors to respond to the Shh signal. Whole-embryo culture experiments indicate that the proper response of cells to Shh requires two RA-dependent mechanisms: (1) a balanced antagonism between Fgf and RA signals, and (2) a RA-mediated repression of Gli2 expression. Thus, an interplay between RA, Fgf and Shh signalling is likely to be an important mechanism underpinning the tight regulation of caudal embryonic development. |
| Databáze: | OpenAIRE |
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