Ultrastructure of sonic and digitonin fragments from beef heart mitochondria
| Autor: | A.N. Malviya, Bahram Parsa, Ralph E. Yodaiken, W.B. Elliott |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
endocrine system
Biophysics Hydroxybutyrates macromolecular substances Biology Vibration Biochemistry law.invention Electron Transport Complex IV chemistry.chemical_compound Oxygen Consumption law polycyclic compounds Animals Inner membrane Membranes Staining and Labeling Histocytochemistry Myocardium Vesicle Oxides Succinates Cell Biology respiratory system Osmium Negative stain Mitochondria Muscle carbohydrates (lipids) Microscopy Electron Digitonin Membrane chemistry Ultrastructure Cardanolides Cattle Electron microscope Polarography |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Bioenergetics. 162:195-209 |
| ISSN: | 0005-2728 |
| Popis: | Examination of OsO4-fixed, imbedded and sectioned specimens by the electron microscope showed that both sonic and digitonin fragments of mitochondria were vesicular structures. Brief OsO4 fixation followed by negative staining showed that most of the ‘inner membrane subunits’ of sonic fragments were on the exterior of the fragment while most of the inner membrane subunits of digitonin fragments were on the inside of the vesicle. Since the orientation of the membrane in the digitonin fragments is the same as that in intact mitochondria, it is not surprising that many properties of digitonin fragments are quite similar to those of mitochondria while sonic fragments show quite different behavior. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|