Insights into the skin microbiome of sickle cell disease leg ulcers
Autor: | Kyle Bittinger, Katherine D. Blizinsky, Julia Byeon, Keisha Findley, Caterina P. Minniti, Jung-Jin Lee, Anitra Persaud, Ashley Buscetta, Vence L. Bonham, Elizabeth A. Grice, Shuo You |
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Rok vydání: | 2021 |
Předmět: |
Chronic wound
food.ingredient Finegoldia Anemia Sickle Cell Dermatology Disease medicine.disease_cause Original Research‐Basic Science 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine food Lactobacillus medicine Humans Microbiome 16S rRNA chronic wound Wound Healing biology business.industry Microbiota Leg Ulcer biology.organism_classification Pathophysiology Cross-Sectional Studies Immunology sickle cell disease skin microbiome Surgery Anaerobic bacteria medicine.symptom business Staphylococcus |
Zdroj: | Wound Repair and Regeneration |
ISSN: | 1524-475X 1067-1927 |
Popis: | Leg ulcers are estimated to occur in 1%–10% of North American patients with sickle cell disease (SCD). Their pathophysiology remains poorly defined, but as with other chronic wounds, it is hypothesised that the microbial milieu, or microbiome, contributes to their healing and clinical outcomes. This study utilises 16S ribosomal RNA (rRNA) gene sequencing to describe, for the first time, the microbiome of the SCD leg ulcer and its association with clinical factors. In a cross‐sectional analysis of 42 ulcers, we recovered microbial profiles similar to other chronic wounds in the predominance of anaerobic bacteria and opportunistic pathogens including Staphylococcus, Corynebacterium, and Finegoldia. Ulcers separated into two clusters: one defined by predominance of Staphylococcus and smaller surface area, and the other displaying a greater diversity of taxa and larger surface area. We also find that the relative abundance of Porphyromonas is negatively associated with haemoglobin levels, a key clinical severity indicator for SCD, and that Finegoldia relative abundance is negatively associated with CD19+ B cell count. Finally, ratios of Corynebacterium:Lactobacillus and Staphylococcus:Lactobacillus are elevated in the intact skin of individuals with a history of SCD leg ulcers, while the ratio of Lactobacillus:Bacillus is elevated in that of individuals without a history of ulcers. Investigations of the skin microbiome in relation to SCD ulcer pathophysiology can inform clinical guidelines for this poorly understood chronic wound, as well as enhance broader understanding about the role of the skin microbiome in delayed wound healing. |
Databáze: | OpenAIRE |
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