Colocalisation of vascular endothelial growth factor and its Flt-1 receptor in human placenta
| Autor: | Xiao F. Li, Martin J. Whittle, D. Ian Rushton, Asif Ahmed, Terry Rollason, Caroline Dunk |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Vascular Endothelial Growth Factor A
medicine.medical_treatment Placenta Clinical Biochemistry Endothelial Growth Factors Andrology chemistry.chemical_compound Endocrinology Syncytiotrophoblast Pregnancy Proto-Oncogene Proteins medicine Decidua Humans Decidual cells reproductive and urinary physiology Cells Cultured Lymphokines Cytotrophoblast Vascular Endothelial Growth Factor Receptor-1 Chemistry Vascular Endothelial Growth Factors Growth factor Parathyroid Hormone-Related Protein Trophoblast Proteins Receptor Protein-Tyrosine Kinases Cell Differentiation Cell Biology Immunohistochemistry Trophoblasts Vascular endothelial growth factor Pregnancy Trimester First medicine.anatomical_structure embryonic structures Immunology Female Immunoradiometric Assay Immunostaining Cell Division |
| Zdroj: | Growth factors (Chur, Switzerland). 12(3) |
| ISSN: | 0897-7194 |
| Popis: | Vascular endothelial growth factor (VEGF) is an angiogenic protein which acts on both endothelial and trophoblast cells. In first trimester placenta, VEGF immunoreactive protein was detected in cytotrophoblast shell suggesting a role in the regulation of cytotrophoblast growth and differentiation as they also expressed VEGF receptor (flt-1) protein. VEGF and flt-1 immunoreactive proteins were expressed in Hofbauer cells within the villous mesenchyme, macrophages and in maternal decidual cells while weak VEGF immunoreactive protein was seen in syncytiotrophoblast surrounding the placental villi in first and second trimester placentae. At term, there was relatively weak VEGF and flt-1 immunostaining in the syncytiotrophoblast while intense VEGF immunostaining was seen in the Hofbauer and maternal decidual cells. Extravillous trophoblast showed immunostaining for flt-1 but no staining for VEGF. Both amnion and chorion expressed strong VEGF immunoreactivity throughout gestation. Smooth muscle cells surrounding the vein and arteries of the umbilical cord showed weak VEGF immunoreactivity while no immunoreactivity was localised in endothelial cells. VEGF stimulated parathyroid hormone-related protein (PTHrP) release (mean (+/- SD): basal, 0.96 +/- 0.03; 10 ng/ml VEGF165, 2.07 +/- 0.18 and 20 ng/ml VEGF165, 2.43 +/- 0.18 pmol/l/well of PTHrP1-86) in condition medium from immortalised first trimester trophoblast cell line. These results suggest that VEGF in addition to acting as an autocrine mitogen for trophoblast proliferation may also function as a paracrine mediator of vascular tone by releasing vasorelaxants from trophoblasts. |
| Databáze: | OpenAIRE |
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