Quantifying trophoblast migration: In vitro approaches to address in vivo situations
Autor: | Alys R. Clark, Win Min Tun, Joanna L. James |
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Jazyk: | angličtina |
Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
Cell type Spiral artery Biology Time-lapse microscopy Cell Line Extracellular matrix 03 medical and health sciences Cellular and Molecular Neuroscience In vivo Cell Movement Pregnancy medicine Human Umbilical Vein Endothelial Cells Humans reproductive and urinary physiology Technical Paper Decidua Trophoblast Cell migration Cell Biology Cell biology Trophoblasts 030104 developmental biology medicine.anatomical_structure Immunology embryonic structures Female Cell Migration Assays |
Popis: | When trophoblasts migrate and invade in vivo, they do so by interacting with a range of other cell types, extracellular matrix proteins, chemotactic factors and physical forces such as fluid shear stress. These factors combine to influence overall trophoblast migration and invasion into the decidua, which in turn determines the success of spiral artery remodelling, and pregnancy itself. Our understanding of these important but complex processes is limited by the simplified conditions in which we often study cell migration in vitro, and many discrepancies are observed between different in vitro models in the literature. On top of these experimental considerations, the migration of individual trophoblasts can vary widely. While time-lapse microscopy provides a wealth of information on trophoblast migration, manual tracking of individual cell migration is a time consuming task that ultimately restricts the numbers of cells quantified, and thus the ability to extract meaningful information from the data. However, the development of automated imaging algorithms is likely to aid our ability to accurately interpret trophoblast migration in vitro, and better allow us to relate these observations to in vivo scenarios. This commentary discusses the advantages and disadvantages of techniques commonly used to quantify trophoblast migration and invasion, both from a cell biology and a mathematical perspective, and examines how such techniques could be improved to help us relate trophoblast migration more accurately to in vivo function in the future. |
Databáze: | OpenAIRE |
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