HIV-1 in lymph nodes is maintained by cellular proliferation during antiretroviral therapy
| Autor: | Michael J. Bale, Andrew Musick, Xiaolin Wu, John M. Coffin, Joshua C. Cyktor, Elizabeth M. Anderson, Sean C Patro, Brandon F. Keele, Elias K. Halvas, Ann Wiegand, Steven G. Deeks, Jeffrey M. Milush, Stephen H. Hughes, Michele D. Sobolewski, Jonathan Spindler, William R McManus, Wei Shao, Rebecca Hoh, Mary F. Kearney, John W. Mellors, Victoria K Musick, Daria Wells |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Gene Expression Regulation Viral Male Population Immunology Human immunodeficiency virus (HIV) T cells HIV Infections Biology medicine.disease_cause Virus Replication Medical and Health Sciences AIDS/HIV 03 medical and health sciences 0302 clinical medicine Clinical Research medicine 2.1 Biological and endogenous factors Humans Viral rna Viral Aetiology education Lymph node Cell Proliferation education.field_of_study General Medicine Single sequence Virology Antiretroviral therapy 030104 developmental biology medicine.anatomical_structure Infectious Diseases Viral replication Gene Expression Regulation Anti-Retroviral Agents 030220 oncology & carcinogenesis HIV-1 RNA HIV/AIDS RNA Viral Female Lymph Lymph Nodes Infection Research Article Follow-Up Studies |
| Zdroj: | The Journal of clinical investigation, vol 129, iss 11 |
| Popis: | To investigate the possibility that HIV-1 replication in lymph nodes sustains the reservoir during antiretroviral therapy (ART), we looked for evidence of viral replication in 5 donors after up to 13 years of viral suppression. We characterized proviral populations in lymph nodes and peripheral blood before and during ART, evaluated the levels of viral RNA expression in single lymph node and blood cells, and characterized the proviral integration sites in paired lymph node and blood samples. Proviruses with identical sequences, identical integration sites, and similar levels of RNA expression were found in lymph nodes and blood samples collected during ART, and no single sequence with significant divergence from the pretherapy population was detected in either blood or lymph nodes. These findings show that all detectable persistent HIV-1 infection is consistent with maintenance in lymph nodes by clonal proliferation of cells infected before ART and not by ongoing viral replication during ART. |
| Databáze: | OpenAIRE |
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