The biosynthetic relationship between littorine and hyoscyamine in transformed roots of Datura stramonium

Autor:	John T. G. Hamilton, David O'Hagan, Ioannis Zabetakis, Robert Edwards
Rok vydání:	2019
Předmět:	Datura stramonium Oxidase test biology Plant Science General Medicine Metabolic intermediate biology.organism_classification Littorine chemistry.chemical_compound Biochemistry Biosynthesis chemistry medicine Secondary metabolism Agronomy and Crop Science Solanaceae Hyoscyamine medicine.drug
Zdroj:	Plant cell reports. 18(3-4)
ISSN:	1432-203X
Popis:	The metabolic relationship between littorine and hyoscyamine has been monitored in transformed roots of Datura stramonium. Quantification by GC of unlabelled littorine and by GCMS of 13C-labelled littorine demonstrated that exogenously added littorine (0.1 mM) was significantly metabolised (35%) to hyoscyamine. In contrast, exogenously added hyoscyamine was not metabolised to littorine, indicating that this conversion is irreversible. The conversion of littorine to hyoscyamine was suppressed by P-450 oxidase inhibitors (particularly clotrimazole), implicating the involvement, at least in part of a cytochrome P-450 activity operating hyoscyamine biosynthesis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97eb963b171161b5a12bf4c742e2fcae https://pubmed.ncbi.nlm.nih.gov/30744247 Zobrazit plný text záznamu Full text from SpringerLink