Fluorescence resonance energy transfer visualization of DNA aggregates formed during electrophoretic separations in ultradilute hydroxyethylcellulose
| Autor: | Jeffrey J. Schwinefus, Michael D. Morris, Shau Chun Wang, Richard W. Hammond |
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| Rok vydání: | 1996 |
| Předmět: |
Electrophoresis
Chemistry Energy transfer Clinical Biochemistry Analytical chemistry Biochemistry Analytical Chemistry chemistry.chemical_compound Spectrometry Fluorescence Förster resonance energy transfer Capillary electrophoresis Microscopy Fluorescence Myoviridae DNA Viral Microscopy Compact form Biophysics Nucleic Acid Conformation Cellulose DNA |
| Zdroj: | Electrophoresis. 17:1110-1114 |
| ISSN: | 1522-2683 0173-0835 |
| DOI: | 10.1002/elps.1150170621 |
| Popis: | Fluorescence resonance energy transfer (FRET) microscopy is used to observe structures of DNA aggregates formed during electrophoresis in ultradilute hydroxyethylcellulose (HEC). FRET gives evidence of aggregates with a tightly packed core and random entanglement of DNA in the outer portions. HEC concentrations also appear to play a role in aggregate formation. DNA aggregates take longer to assemble in higher HEC concentration solutions and assume a less compact form than those formed at lower HEC concentrations. |
| Databáze: | OpenAIRE |
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