Detection of bacterial biofilm on cochlear implants removed because of device failure, without evidence of infection
| Autor: | Patrice Tran Ba Huy, Philippe Herman, Romain Kania, Thierry Van Den Abbeele, Gerda E. M. Lamers, Charlotte Hautefort, Guido V. Bloemberg, Johan H. M. Frijns, Katell Ruellan |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Male medicine.medical_specialty Prosthesis-Related Infections Confocal Audiology Microscopy Electrode array Image Processing Computer-Assisted Medicine Humans Prospective Studies Prosthesis-Related Infection Child Device failure Aged Microscopy Confocal business.industry Outcome measures Biofilm Biofilms Biomaterials infection Cochlear implants Meningitis in-vitro model otitis-media middle-ear children meningitis management risk Middle Aged Sensory Systems medicine.anatomical_structure Cochlear Implants Otorhinolaryngology Biofilms Child Preschool Middle ear Microscopy Electron Scanning Equipment Failure Female Neurology (clinical) business Biomedical engineering |
| Zdroj: | Otology and Neurotology, 31(8), 1320-1324 |
| ISSN: | 1537-4505 |
| Popis: | Objective: To investigate the formation of bacterial biofilms on the surface of the electrode array of cochlear implants (CI) explanted because of device failure, without evidence of infection, by use of scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Study Design: Prospective study. Setting: Patients from 2 tertiary-care referral centers. Patients and Methods: CIs were explanted from 9 patients because of device failure. Specimens were immediately snap-frozen in cold isopenthane, stored at −80°C and examined with SEM and CLSM by 3 investigators. Main Outcome Measure: Presence of bacterial biofilm ascertained by SEM and CSLM. Results: One specimen showed the formation of a bacterial biofilm on the middle ear part of the electrode array. No biofilm formation was found in the inner-ear part of electrode arrays. In the middle-ear part of the electrode array, a cylindrical cover of human muscular tissue was seen plugging the cochleostomy. Conclusion: This is the first study demonstrating that bacterial biofilms may exist on the surface of the electrode array of CIs explanted because of device failure but not infection. We found 1 case of biofilm formation in 9 explanted CIs. Further studies with larger series of CIs are required to investigate biofilm formation on the surface of CI electrode arrays to address both the pathophysiology of bacterial biofilms and prevention of device-related infections in CI patients. |
| Databáze: | OpenAIRE |
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