Akt1Controls the Timing and Amplitude of Vascular Circadian Gene Expression
| Autor: | William C. Sessa, Heino Velazquez, Amelia K. Luciano, Jeans M. Santana |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gene isoform Physiology AKT1 Biology Article Mice 03 medical and health sciences 0302 clinical medicine Transcription (biology) Physiology (medical) Gene expression Animals Protein Isoforms Circadian rhythm Protein kinase B Aorta Mice Knockout Akt/PKB signaling pathway Kinase Endothelial Cells Circadian Rhythm Cell biology 030104 developmental biology Gene Expression Regulation Liver embryonic structures Proto-Oncogene Proteins c-akt 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Journal of Biological Rhythms. 32:212-221 |
| ISSN: | 1552-4531 0748-7304 |
| DOI: | 10.1177/0748730417704534 |
| Popis: | The AKT signaling pathway is important for circadian rhythms in mammals and flies ( Drosophila). However, AKT signaling in mammals is more complicated since there are 3 isoforms of AKT, each performing slightly different functions. Here we study the most ubiquitous AKT isoform, Akt1, and its role at the organismal level in the central and vascular peripheral clocks. Akt1-/- mice exhibit relatively normal behavioral rhythms with only minor differences in circadian gene expression in the liver and heart. However, circadian gene expression in the Akt1-/- aorta, compared with control aorta, follows a distinct pattern. In the Akt1-/- aorta, positive regulators of circadian transcription have lower amplitude rhythms and peak earlier in the day, and negative circadian regulators are expressed at higher amplitudes and peak later in the day. In endothelial cells, negative circadian regulators exhibit an increased amplitude of expression, while the positive circadian regulators are arrhythmic with a decreased amplitude of expression. This indicates that Akt1 conditions the normal circadian rhythm in the vasculature more so than in other peripheral tissues where other AKT isoforms or kinases might be important for daily rhythms. |
| Databáze: | OpenAIRE |
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