Keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile
| Autor: | Archana Padmanabhan Nair, Swaminathan Sethu, Ganesh Ram Sahu, Mor M. Dickman, Tanuja Vaidya, Rohit Shetty, Arkasubhra Ghosh, Ritika Mullick, Rudy M.M.A. Nuijts, Sharon D'Souza, Rajiv R. Mohan, Pooja Khamar, Sneha Gupta |
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| Přispěvatelé: | MUMC+: MA UECM Oogartsen MUMC (9), RS: MHeNs - R3 - Neuroscience, MUMC+: MA UECM Oogartsen ZL (9), MUMC+: MA UECM AIOS (9), Oogheelkunde, MUMC+: *AB Refractie Chirurgie Oogheelkunde (9) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
TEAR FILM Diseases Immunoglobulin E Eye Pathogenesis DELTA T-CELLS LYSYL OXIDASE OXIDATIVE STRESS Multidisciplinary biology medicine.diagnostic_test Molecular medicine Natural killer T cell Cytokines Medicine Tumor necrosis factor alpha Female Adult EXPRESSION Keratoconus Science Immunology EPITHELIUM Enzyme-Linked Immunosorbent Assay Article Flow cytometry Young Adult Immune system Medical research medicine Hypersensitivity EXTRACELLULAR-MATRIX Humans Inflammation business.industry MATRIX-METALLOPROTEINASE-9 GAMMA medicine.disease COLLAGEN eye diseases Cross-Sectional Studies Perforin Immune System Tears biology.protein business Biomarkers |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021) Scientific Reports, 11(1):20891. Nature Publishing Group Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Inflammatory factors have been considered to contribute to keratoconus (KC) pathogenesis. This study aims to determine the immune cells subsets and soluble inflammatory factor profile on the ocular surface of KC patients. 32 KC subjects (51 eyes) across different grades of severity and 15 healthy controls (23 eyes) were included in the study. Keratometry and pachymetry measurements were recorded. Ocular surface immune cells (collected by ocular surface wash) immunophenotyped using flow cytometry include leukocytes, neutrophils, macrophages, natural killer (NK) cells, pan-T cells, gamma delta T (gamma delta T) cells and NKT cells. Tear fluid collected using Schirmer's strip was used to measure 50 soluble factors by multiplex ELISA. Proportions of activated neutrophils, NK cells and gamma delta T cells were significantly increased in KC patients. Significantly higher levels of tear fluid IL-1 beta, IL-6, LIF, IL-17A, TNF alpha, IFN alpha/beta/gamma, EPO, TGF beta 1, PDGF-BB, sVCAM, sL-selectin, granzyme-B, perforin, MMP2, sFasL and IgE, along with significantly lower levels of IL-1 alpha and IL-9 were observed in KC patients. Alterations observed in few of the immuno-inflammatory parameters correlated with grades of disease, allergy, eye rubbing and keratometry or pachymetry measurements. The observation implies a distinct immuno-inflammatory component in KC pathogenesis and its potential as an additional therapeutic target in KC management. |
| Databáze: | OpenAIRE |
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