Influence of Different Irradiation Protocols on Vascularization and Bone Formation Parameters in Rat Femora
| Autor: | Raymund E. Horch, Ulrike Rottensteiner-Brandl, Andreas Arkudas, Martin Stumpf, Peter Greil, Ulf Bertram, Lara F. Lingens, Luitpold Distel, Tobias Fey, Katrin Köhn |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Angiogenesis Biomedical Engineering Neovascularization Physiologic Medicine (miscellaneous) Bioengineering Bone healing Real-Time Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine Osteogenesis medicine Animals Femur Staining and Labeling biology Chemistry X-Rays Histology X-Ray Microtomography Anatomy Hypoxia-Inducible Factor 1 alpha Subunit Vascular endothelial growth factor A 030104 developmental biology medicine.anatomical_structure Rats Inbred Lew 030220 oncology & carcinogenesis Osteocalcin biology.protein Cortical bone Bone marrow |
| Zdroj: | Tissue Engineering Part C: Methods. 23:583-591 |
| ISSN: | 1937-3392 1937-3384 |
| DOI: | 10.1089/ten.tec.2017.0170 |
| Popis: | Aim of the present study was the establishment of an efficient and reproducible model for irradiation of rat femora as a model for impaired osteogenesis and angiogenesis. Four different irradiation protocols were compared: single irradiation of the left femur with 20 Gy and explantation after 4 or 8 weeks (group A, B) and three irradiation fractions at 3-4 days intervals with 10 Gy and explantation after 4 or 8 weeks (group C, D). The contralateral, unirradiated femur served as control. Evaluation included histology, microcomputertomography (μCT), and real-time polymerase chain reaction. Histology showed a pronounced increase of vacuoles in bone marrow after irradiation, especially after 4 weeks (group A and C), demonstrating bone marrow edema and fatty degeneration. Irradiation provoked a decrease of total cell numbers in cortical bone and of hypoxia-inducible factor 1 alpha (HIF1α)-positive cells in bone marrow. The expression of several markers (osteocalcin [OCN], runt-related transcription factor 2 [RUNX2], transforming growth factor beta 1 [TGFβ1], tumor necrosis factor alpha [TNFα], vascular endothelial growth factor A [VEGFA], and HIF1α) was decreased in group A after irradiation. This might suggest a decreased metabolism after irradiation. A significant decrease in small-sized vessels was seen in μCT evaluation in group A and D. Single irradiation with 20 Gy had the most severe and reproducible impact on osteogenesis and angiogenesis after 4 weeks while being well tolerated by all animals, thus making it an excellent model for evaluation of bone healing and vascularization in irradiated tissue. |
| Databáze: | OpenAIRE |
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