An immunoglobulin promoter displays cell-type specificity independently of the enhancer
| Autor: | Jeannine Stafford, John Lewis Foster, Cary Queen |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Immunoglobulin gene
Transcription Genetic DNA Recombinant Biology Immunoglobulin kappa-Chains Mice Transcription (biology) Genes Regulator Gene expression Animals Tissue Distribution Lymphocytes RNA Messenger Promoter Regions Genetic Enhancer Gene Cells Cultured Multidisciplinary Promoter Transfection Molecular biology Enhancer Elements Genetic Gene Expression Regulation biology.protein Immunoglobulin Light Chains Antibody |
| Zdroj: | Nature. 315:423-425 |
| ISSN: | 1476-4687 0028-0836 |
| DOI: | 10.1038/315423a0 |
| Popis: | Recent studies in which cloned immunoglobulin genes were introduced into cultured cells have produced two significant findings. First, the genes are expressed after transfection into lymphoid cells but not non-lymphoid cells1–3. Second, transcription of an immunoglobulin gene requires, in addition to the promoter region, an enhancer element located downstream of the transcription start site4–10. These findings raise the question of whether it is the promoter or the enhancer region that is responsible for the observed cell-type specificity. It has, in fact, been shown that immunoglobulin enhancers function only in lymphoid cells6,8,9. We show here that the promoter for an immunoglobulin κ light-chain gene also is strongly specific for lymphoid cells. Our result re-emphasizes the importance of promoters relative to enhancers in determining which cells express which genes. |
| Databáze: | OpenAIRE |
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