A genetically encoded sensor with improved fluorescence intensity for opioid detection at cellular resolution
| Autor: | Wenjing Wang, D. Isabel Petrescu, Mingcheng Li, Kayla E. Kroning |
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| Rok vydání: | 2021 |
| Předmět: |
Models
Molecular Fluorophore Green Fluorescent Proteins Receptors Opioid mu Catalysis Fluorescence Green fluorescent protein chemistry.chemical_compound Materials Chemistry medicine Humans Receptor Cells Cultured chemistry.chemical_classification Chemistry HEK 293 cells Metals and Alloys General Chemistry Surfaces Coatings and Films Electronic Optical and Magnetic Materials Amino acid Cell biology HEK293 Cells Cellular resolution Opioid Cell culture Ceramics and Composites medicine.drug |
| Zdroj: | Chemical communications (Cambridge, England). 57(81) |
| ISSN: | 1364-548X |
| Popis: | The mu-opioid receptor (MOR) regulates the neuronal pathways involved in pain, reward, and respiration. To increase our understanding of MOR's roles in these pathways, there is a need to detect opioids at cellular resolution. Here, we engineered an improved opioid-sensor, called M-SPOTIT2, which is 11x brighter than our previously engineered M-SPOTIT1.1. We engineered M-SPOTIT2 by adding the amino acids YNSH, located near the fluorophore of the enhanced green fluorescent protein, to the circular permuted green fluorescent protein in M-SPOTIT2. M-SPOTIT2 is 11x brighter than our previously engineered M-SPOTIT1.1 in HEK293T cell culture and 2.7x brighter in neuronal culture. M-SPOTIT2 will potentially be useful for the detection of opioids in cell culture for drug screening and the detection of opioids at cellular resolution in animal tissues. By using M-SPOTIT2, researchers can gain more understanding about the mechanisms of addiction, respiratory suppression, and pain-modulation involved in opioid signaling. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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