| Popis: |
Figure S1 - PDF file 105K, Figure S1. Neuroblastoma cell lines do not express 4-1BB, CD40 or CTLA-4. The expression of 4-1BB, CD40 and CTLA-4 on naive and activated splenocytes (0.5 mg anti-CD3 i.v. 48 hr) and the neuroblastoma cell lines was determined by indirect flow cytometry. Cells were incubated with 10 microg/ml unlabelled mAb, either isotype control (Mc39/16) anti-4-1BB (LOB12.3), anti-CD40 (3/23) or anti-CTLA-4 (UC10 4F10-11) prior to washing and incubation with PE-conjugated anti-mouse Fc. Expression of 4-1BB, CD40 and CTLA-4 could be observed on T-cells from activated splenocytes, but not on naive T-cells as expected. Expression of 4-1BB, CD40 and CTLA-4 was not evident on Neuro2a, AgN2a or NXS2 cell lines. Data represents an example of three experiments |
