The Propagation, Quantification, and Storage of Vesicular Stomatitis Virus
| Autor: | Alaa A. Abdelmageed, Maureen C. Ferran |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Virus Cultivation
viruses Preservation Biological Viral Plaque Assay Microbiology Vesicular stomatitis Indiana virus Article Virus Cell Line Specimen Handling 03 medical and health sciences Virology Biosafety level Chlorocebus aethiops Animals Humans Vero Cells Pathogen 030304 developmental biology Virus quantification 0303 health sciences biology 030306 microbiology Rhabdoviridae biology.organism_classification Oncolytic virus Vesicular stomatitis virus Vero cell Parasitology Vesicular Stomatitis |
| Zdroj: | Curr Protoc Microbiol |
| ISSN: | 1934-8533 1934-8525 |
| Popis: | Vesicular stomatitis virus (VSV) is the prototypical member of the Rhabdoviridae family of negative-sense single-stranded RNA viruses. This virus has been used as a powerful model system for decades and is currently being used as a vaccine platform and an oncolytic agent. Here, we present methods to propagate, quantitate, and store VSV. We also review the proper safety protocol for the handling of VSV, which is classified as a Biosafety Level 2 pathogen by the United States Centers for Disease Control and Prevention. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Generation, purification, and storage of vesicular stomatitis virus stocks Basic Protocol 2: Quantification of vesicular stomatitis virus by plaque assay Support Protocol: Propagation of Vero cells. |
| Databáze: | OpenAIRE |
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