Influence of cell adhesion and spreading on impedance characteristics of cell-based sensors
| Autor: | Ryan Kosai, Myo Thein, Omid Veiseh, Mandana Veiseh, Dennis Edmondson, Fareid Asphahani, Miqin Zhang, Jian Xu |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Materials science
Biomedical Engineering Biophysics Nanotechnology Electrochemistry Cell morphology Sensitivity and Specificity Article Mice Cell Movement Cell Adhesion Electric Impedance Animals Cell adhesion Reproducibility of Results General Medicine Adhesion Equipment Design Cell Adhesion Process Equipment Failure Analysis Microelectrode Electrode NIH 3T3 Cells Biological Assay Biosensor Microelectrodes Biotechnology |
| Popis: | Impedance measurements of cell-based sensors are a primary characterization route for detection and analysis of cellular responses to chemical and biological agents in real time. The detection sensitivity and limitation depend on sensor impedance characteristics and thus on cell patterning techniques. This study introduces a cell patterning approach to bind cells on microarrays of gold electrodes and demonstrates that single-cell patterning can substantially improve impedance characteristics of cell-based sensors. Mouse fibroblast cells (NIH3T3) are immobilized on electrodes through a lysine-arginine-glycine-aspartic acid (KRGD) peptide-mediated natural cell adhesion process. Electrodes are made of three sizes and immobilized with either covalently bound or physically adsorbed KRGD (c-electrodes or p-electrodes). Cells attached to c-electrodes increase the measurable electrical signal strength by 48.4%, 24.2%, and 19.0% for three electrode sizes, respectively, as compared to cells attached to p-electrodes, demonstrating that both the electrode size and surface chemistry play a key role in cell adhesion and spreading and thus the impedance characteristics of cell-based sensors. Single cells patterned on c-electrodes with dimensions comparable to cell size exhibit well-spread cell morphology and substantially outperform cells patterned on electrodes of other configurations. |
| Databáze: | OpenAIRE |
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