Responses of peripheral blood mononucleated cells from non-celiac gluten sensitive patients to various cereal sources
| Autor: | Raffaella Di Silvestro, Luigia De Fazio, C Ricci, Massimo Campieri, Barbara Pavan, Giovanni Dinelli, Alessandro Dalpiaz, Alberto Lanzini, Enzo Spisni, Elena Cavazza, Umberto Volta, Maria Chiara Valerii |
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| Přispěvatelé: | Valerii, Maria Chiara, Ricci, Chiara, Spisni, Enzo, Silvestro, Raffaella Di, De Fazio, Luigia, Cavazza, Elena, Lanzini, Alberto, Campieri, Massimo, Dalpiaz, Alessandro, Pavan, Barbara, Volta, Umberto, Dinelli, Giovanni |
| Jazyk: | angličtina |
| Předmět: |
Adult
Male Chemokine Glutens ncient grains CXCL10 Non celiac gluten sensitivity Wheat proteins Peripheral blood mononuclear cell NO Proinflammatory cytokine Analytical Chemistry Autoimmune Diseases Diet Gluten-Free Young Adult Gene expression Humans Triticum Aged chemistry.chemical_classification biology Tight junction General Medicine Middle Aged Gluten Celiac Disease chemistry Tight junction protein 2 Immunology biology.protein Leukocytes Mononuclear Wheat proteins Non celiac gluten sensitivity CXCL10 Ancient grains Edible Grain Wheat protein Food Hypersensitivity Ancient grains Food Science |
| Zdroj: | Food Chemistry. :167-174 |
| ISSN: | 0308-8146 |
| DOI: | 10.1016/j.foodchem.2014.12.061 |
| Popis: | Non-celiac gluten sensitivity (NCGS) is still an undefined syndrome whose triggering mechanisms remain unsettled. This study aimed to clarify how cultured peripheral blood mononucleated cells (PBMC) obtained from NCGS patients responded to contact with wheat proteins. Results demonstrated that wheat protein induced an overactivation of the proinflammatory chemokine CXCL10 in PBMC from NCGS patients, and that the overactivation level depends on the cereal source from which proteins are obtained. CXCL10 is able to decrease the transepithelial resistance of monolayers of normal colonocytes (NCM 460) by diminishing the mRNA expression of cadherin-1 (CDH1) and tight junction protein 2 (TJP2), two primary components of the tight junction strands. Thus, CXCL10 overactivation is one of the mechanisms triggered by wheat proteins in PBMC obtained from NCGS patients. This mechanism is activated to a greater extent by proteins from modern with respect to those extracted from ancient wheat genotypes. |
| Databáze: | OpenAIRE |
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