A rapid test for the environmental detection of pigeon antigen
Autor: | Silvia Sánchez-Díez, Jeroen Vanoirbeek, Peter Hoet, Susana Gómez-Ollés, Daniel Alvarez-Simón, María Jesús Cruz, Tomás Montalvo, Xavier Muñoz |
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Rok vydání: | 2021 |
Předmět: |
Environmental Engineering
010504 meteorology & atmospheric sciences Immunochromatographic test INDOOR Environmental Sciences & Ecology Enzyme-Linked Immunosorbent Assay 010501 environmental sciences 01 natural sciences Rapid detection Chromatography Affinity Article SERUM Immunoenzyme Techniques Kappa index Pigeon antigen Antigen parasitic diseases medicine Animals Environmental Chemistry Elisa method Columbidae Waste Management and Disposal 0105 earth and related environmental sciences Immunoassay Science & Technology Chromatography biology medicine.diagnostic_test PULMONARY-FIBROSIS Disease progression Pollution 3. Good health Strip assay biology.protein CHRONIC HYPERSENSITIVITY PNEUMONITIS AVIAN ANTIGEN Antibody Life Sciences & Biomedicine Environmental Sciences Hypersensitivity pneumonitis |
Zdroj: | Science of The Total Environment Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona The Science of the Total Environment |
ISSN: | 0048-9697 |
DOI: | 10.1016/j.scitotenv.2021.147789 |
Popis: | Introduction Avoidance of inhaled bird antigens is essential to prevent hypersensitivity pneumonitis disease progression. The aim of the present study was to develop a sandwich enzyme link immunoassay (ELISA) and an immunochromatographic test (ICT) and compare their ability to detect pigeon antigens in environmental samples. Methods An amplified sandwich ELISA using pigeon serum as a calibration standard and a ICT using gold-labeled anti-pigeon serum antibodies for the rapid detection of pigeon antigens in environmental samples were developed. Twenty-two different airborne samples were collected and analysed using both methods. Strip density values obtained with ICT were calculated and compared with the concentrations determined by the ELISA method for pigeon antigens. Strips results were also visually analysed by five independent evaluators. Results The ELISA method to quantify pigeon antigen had a broader range (58.4 and 10,112.2 ng/ml), compared to the ICT assay (420 to 3360 ng/ml). A kappa index of 0.736 (p < 0.0001) was obtained between the observers evaluating the ICT strips. The results of the ELISA and the relative density of the ICT showed a highly significant correlation (rs:0.935; p < 0.0001). Bland-Altman plot also confirmed excellent agreement between the two methods (mean difference: −1.626; p < 0.0001). Conclusions Since there was a good correlation between both assays, we can conclude that the rapid and simple ICT assay is a good and valid alternative, which does not require expensive equipment, for the validated ELISA technique. Graphical abstract Unlabelled Image Highlights • Demonstrating antigen exposure is critical in the diagnosis and management of hypersensitivity pneumonitis. • An immunochromatographic test is compared with an ELISA method for the measurement of pigeon antigens in the environment. • The immunochromatographic test described was highly consistent with the validated ELISA and does not require specific skills. • This method may prove to be a useful tool for predicting the progression of bird-related hypersensitivity pneumonitis. |
Databáze: | OpenAIRE |
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