A rapid test for the environmental detection of pigeon antigen

Autor: Silvia Sánchez-Díez, Jeroen Vanoirbeek, Peter Hoet, Susana Gómez-Ollés, Daniel Alvarez-Simón, María Jesús Cruz, Tomás Montalvo, Xavier Muñoz
Rok vydání: 2021
Předmět:
Environmental Engineering
010504 meteorology & atmospheric sciences
Immunochromatographic test
INDOOR
Environmental Sciences & Ecology
Enzyme-Linked Immunosorbent Assay
010501 environmental sciences
01 natural sciences
Rapid detection
Chromatography
Affinity
Article
SERUM
Immunoenzyme Techniques
Kappa index
Pigeon antigen
Antigen
parasitic diseases
medicine
Animals
Environmental Chemistry
Elisa method
Columbidae
Waste Management and Disposal
0105 earth and related environmental sciences
Immunoassay
Science & Technology
Chromatography
biology
medicine.diagnostic_test
PULMONARY-FIBROSIS
Disease progression
Pollution
3. Good health
Strip assay
biology.protein
CHRONIC HYPERSENSITIVITY PNEUMONITIS
AVIAN ANTIGEN
Antibody
Life Sciences & Biomedicine
Environmental Sciences
Hypersensitivity pneumonitis
Zdroj: Science of The Total Environment
Dipòsit Digital de Documents de la UAB
Universitat Autònoma de Barcelona
The Science of the Total Environment
ISSN: 0048-9697
DOI: 10.1016/j.scitotenv.2021.147789
Popis: Introduction Avoidance of inhaled bird antigens is essential to prevent hypersensitivity pneumonitis disease progression. The aim of the present study was to develop a sandwich enzyme link immunoassay (ELISA) and an immunochromatographic test (ICT) and compare their ability to detect pigeon antigens in environmental samples. Methods An amplified sandwich ELISA using pigeon serum as a calibration standard and a ICT using gold-labeled anti-pigeon serum antibodies for the rapid detection of pigeon antigens in environmental samples were developed. Twenty-two different airborne samples were collected and analysed using both methods. Strip density values obtained with ICT were calculated and compared with the concentrations determined by the ELISA method for pigeon antigens. Strips results were also visually analysed by five independent evaluators. Results The ELISA method to quantify pigeon antigen had a broader range (58.4 and 10,112.2 ng/ml), compared to the ICT assay (420 to 3360 ng/ml). A kappa index of 0.736 (p < 0.0001) was obtained between the observers evaluating the ICT strips. The results of the ELISA and the relative density of the ICT showed a highly significant correlation (rs:0.935; p < 0.0001). Bland-Altman plot also confirmed excellent agreement between the two methods (mean difference: −1.626; p < 0.0001). Conclusions Since there was a good correlation between both assays, we can conclude that the rapid and simple ICT assay is a good and valid alternative, which does not require expensive equipment, for the validated ELISA technique.
Graphical abstract Unlabelled Image
Highlights • Demonstrating antigen exposure is critical in the diagnosis and management of hypersensitivity pneumonitis. • An immunochromatographic test is compared with an ELISA method for the measurement of pigeon antigens in the environment. • The immunochromatographic test described was highly consistent with the validated ELISA and does not require specific skills. • This method may prove to be a useful tool for predicting the progression of bird-related hypersensitivity pneumonitis.
Databáze: OpenAIRE
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