A new and fast technique to generate offspring after germ cells transplantation in adult fish: the Nile tilapia (Oreochromis niloticus) model
| Autor: | T. M. Segatelli, Sergio Ricardo Batlouni, Luiz R. França, Guilherme M.J. Costa, Bruno R. Quirino, Samyra M.S.N. Lacerda, Bruno M. Queiroz, Evanguedes Kalapothakis |
|---|---|
| Přispěvatelé: | Universidade Federal de Minas Gerais (UFMG), Universidade Estadual Paulista (Unesp), GM Alevinos LTDA |
| Rok vydání: | 2010 |
| Předmět: |
Male
Aging food.ingredient Developmental Biology/Germ Cells lcsh:Medicine Biology Breeding Models Biological Cryopreservation Andrology Animals Genetically Modified Nile tilapia food Testis medicine Animals lcsh:Science Busulfan Phylogeny Multidisciplinary Reproduction lcsh:R Tilapia Cichlids Cell Biology biology.organism_classification Spermatogonia Physiology/Reproductive Physiology Transplantation Oreochromis medicine.anatomical_structure Immunology lcsh:Q Stem cell Spermatogenesis Germ cell Research Article Biotechnology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 5, p e10740 (2010) Web of Science Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1932-6203 |
| Popis: | Made available in DSpace on 2013-08-28T14:08:22Z (GMT). No. of bitstreams: 1 WOS000278017300013.pdf: 7512680 bytes, checksum: 985d975296b48c7197a127fcd01db2ed (MD5) Made available in DSpace on 2013-09-30T19:22:31Z (GMT). No. of bitstreams: 2 WOS000278017300013.pdf: 7512680 bytes, checksum: 985d975296b48c7197a127fcd01db2ed (MD5) WOS000278017300013.pdf.txt: 44589 bytes, checksum: 9f10ff7988f2d6dbe5fddf41647135fe (MD5) Previous issue date: 2010-05-20 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:33:50Z No. of bitstreams: 2 WOS000278017300013.pdf: 7512680 bytes, checksum: 985d975296b48c7197a127fcd01db2ed (MD5) WOS000278017300013.pdf.txt: 44589 bytes, checksum: 9f10ff7988f2d6dbe5fddf41647135fe (MD5) Made available in DSpace on 2014-05-20T15:33:50Z (GMT). No. of bitstreams: 2 WOS000278017300013.pdf: 7512680 bytes, checksum: 985d975296b48c7197a127fcd01db2ed (MD5) WOS000278017300013.pdf.txt: 44589 bytes, checksum: 9f10ff7988f2d6dbe5fddf41647135fe (MD5) Previous issue date: 2010-05-20 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Background: Germ cell transplantation results in fertile recipients and is the only available approach to functionally investigate the spermatogonial stem cell biology in mammals and probably in other vertebrates. In the current study, we describe a novel non-surgical methodology for efficient spermatogonial transplantation into the testes of adult tilapia (O. niloticus), in which endogenous spermatogenesis had been depleted with the cytostatic drug busulfan.Methodology/Principal Findings: Using two different tilapia strains, the production of fertile spermatozoa with donor characteristics was demonstrated in adult recipient, which also sired progeny with the donor genotype. Also, after cryopreservation tilapia spermatogonial cells were able to differentiate to spermatozoa in the testes of recipient fishes. These findings indicate that injecting germ cells directly into adult testis facilitates and enable fast generation of donor spermatogenesis and offspring compared to previously described methods.Conclusion: Therefore, a new suitable methodology for biotechnological investigations in aquaculture was established, with a high potential to improve the production of commercially valuable fish, generate transgenic animals and preserve endangered fish species. Univ Fed Minas Gerais, Dept Morphol, Lab Cellular Biol, Belo Horizonte, MG, Brazil São Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, Brazil GM Alevinos LTDA, Contagem, MG, Brazil Univ Fed Minas Gerais, Dept Gen Biol, Lab Biotechnol & Mol Markers, Belo Horizonte, MG, Brazil São Paulo State Univ, Aquaculture Ctr CAUNESP, São Paulo, Brazil |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|