Studies on mutagen-sensitive strains of Drosophila melanogaster
| Autor: | W. Ferro |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Genetics
Mutation Strain (chemistry) biology DNA repair Health Toxicology and Mutagenesis Mutant Mutagenesis Embryo Chromosomal translocation Mutagen Toxicology medicine.disease_cause biology.organism_classification Molecular biology chemistry.chemical_compound chemistry Cell culture medicine Drosophila melanogaster Photolyase Molecular Biology DNA Nucleotide excision repair |
| Zdroj: | Mutation Research/DNA Repair Reports. 166:49-57 |
| ISSN: | 0167-8817 |
| DOI: | 10.1016/0167-8817(86)90040-4 |
| Popis: | The influence of defects in DNA repair on the recovery of X-ray-induced genetic damage in spermatozoa of Drosophila melanogaster was studied. Basc males were irradiated in N 2 , air or O 2 and mated to females of 4 repair-deficient mutant types: mus(2)201 D1 (excision repair deficient), mus(3)306 D1 (excision repair deficient), mus(3)302 D1 and mus(3)308 D2 (both excision repair and post-replication repair deficient). The frequencies of sex-lined recessive lethals and of autosomal translocations in the F 1 progeny were determined following standard genetic procedures. The responses in the different crosses with repair-deficient females were compared to those with repair-proficient mei + females (maternal effects). The main findings are the following: (1) with excision repair-deficient females the frequencies of spontaneous recessive lethals tend to be higher than with mei + females; (2) with excision repair-deficient females the frequencies of recessive lethals induced in N 2 and air and often in O 2 are higher than with mei + females; (3) with post-replication repair-deficient mutants a maternal effect is found for X-ray-induced translocations - both increases and decreases occur depending on the specific mutant type. The data are explained as follows: excision repair deficiencies cause the processing of primary lesions to be diverted from the error-free excision repair to the error-prone post-replication repair pathways. This results in enhanced mutational yields. After irradiation in O 2 secondary effects cause selective elimination of potential recessive lethals in those mutants that exhibit lowered fertility ( mei-9 , mus-302 ). Therefore those mutants have no differential maternal effect on the recovery of recessive lethals after irradiation in O 2 . The changes in translocation yield with post-replication repair deficiencies are thought to be the result of defects in the repair of DNA breaks. These defects might cause the post-replication repair deficiency too. The group of post-replication repair mutants is heterogenous. The mutants with low fertility seem to cause decreased translocation frequencies by selective elimination through dominant lethality. The other mutants increase the frequencies. |
| Databáze: | OpenAIRE |
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