HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats
| Autor: | Bature Charles Ushahemba, Glory Ginikachukwu Aninjoku, Chinedu Godwin Uzomba, CO Edeogu, Chukwubuike Cyprian Okoro, Chinyere Aloke, Adeolu Adewale Iyaniwura, Blessing Peter Okey, Ofovwe O. Ekakitie, Chinedum Uche Emelike, Nwogo Ajuka Obasi, Patience Nkemjika Ogbu |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Spleenotoxic effects
Antioxidant medicine.diagnostic_test Chemistry DPPH General Chemical Engineering medicine.medical_treatment General Chemistry Pharmacology Hematocrit medicine.disease_cause chemistry.chemical_compound Phenylhydrazine In vivo Oxidative stress Hematotoxicity medicine Hemoglobin Luteolin QD1-999 Copaifera salikounda |
| Zdroj: | Arabian Journal of Chemistry, Vol 14, Iss 12, Pp 103428-(2021) |
| ISSN: | 1878-5352 |
| Popis: | Anemia is a clinical disorder orchestrated by factors such as drugs, including phenylhydrazine (PHZ). This study explored the protective roles of Copaifera salikounda methanol leaf extract (CSMLE) against PHZ-induced hematotoxicity and oxidative stress in rats. In vitro antioxidants studies of CSMLE using 1,1-diphenyl-2-picryl hydrazyl (DPPH) and ferric reducing antioxidant potential (FRAP) and quantification of CSMLE bioactive compounds using High-Performance-Liquid-Chromatography (HPLC) were done. In vivo, thirty rats in five groups (n = 6) were used. Anemia was induced intraperitoneally in groups 2 to 5 with 40 mg/kg body-weight/day PHZ for two days. Group 1 received normal saline, group 2 (untreated: negative-control), group 3 received 1 ml/kg-body-weight Vit B12 syrup while groups 4 and 5 received 200 mg/kg and 400 mg/kg-body-weight CSMLE, respectively, daily for fourteen days. Result of CSMLE profiling showed luteolin (76.49 ng/Ml) and squalene (38.31 ng/Ml) as the most abundant flavonoid and terpenoid, respectively. In vitro CSMLE demonstrated dose-dependent antioxidant effects on DPPH and FRAP with IC50 of 0.1085 mg/ml and 0.07344 mg/ml (EC50), respectively. In vivo, CSMLE improved the body weight, red blood cell, hematocrit and hemoglobin concentrations, significantly (P |
| Databáze: | OpenAIRE |
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