Antifungal activity of eicosanoic acids isolated from the endophytic fungus Mycosphaerella sp. against Cryptococcus neoformans and C. gattii
| Autor: | Betania Barros Cota, Cristiane B Pereira, Paulo J.S. Barbeira, Susana Johann, Nívea Pereira de Sá, Beatriz M. Borelli, Carlos A. Rosa |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antifungal Agents Hypha 030106 microbiology Cryptococcus Microbial Sensitivity Tests Biology Microbiology 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration Ascomycota Eicosanoic Acids Myriocin Amphotericin B medicine Endophytes Propidium iodide Cryptococcus neoformans Biological Products Cell Membrane Cryptococcus gattii Drug Synergism bacterial infections and mycoses biology.organism_classification 030104 developmental biology Infectious Diseases chemistry medicine.drug |
| Zdroj: | Microbial pathogenesis. 100 |
| ISSN: | 1096-1208 |
| Popis: | The antifungal effects of two eicosanoic acids, 2-amino-3,4-dihydroxy-2-25-(hydroxymethyl)-14-oxo-6,12-eicosenoic acid (compound 1 ) and myriocin (compound 2 ), isolated from Mycosphaerella sp. were evaluated against Cryptococcus neoformans and C. gattii. The compounds displayed antifungal activities against several isolates of C. neoformans and C. gattii, with minimal inhibitory concentration (MIC) values ranging from 0.49 to 7.82 μM for compound 1 and 0.48–1.95 μM for compound 2 . In the checkerboard microtiter test, both compounds exhibited synergistic activity with amphotericin B against C. gattii . Ultrastructural analysis revealed several signs of damage in C. gattii and C. neoformans cells treated with compounds 1 and 2 , including deformities in cell shape, depressions on the surface, and withered cells. The cells of C. gattii treated with compounds 1 and 2 showed less loss of cellular material in comparison to those treated with amphotericin B. The difference in cellular material loss increased in a test compound concentration-dependent manner. Consistent with this observation, compounds 1 and 2 were able to internalize propidium iodide (PI) in C. gattii cells. In addition, compound 2 induced the formation of several pseudohyphae, suggesting that it could reduce virulence in C. gattii cells. The study results show that these natural products led to membrane damage; however, this may not be the main target of action. These compounds have potential antifungal activity and could be useful in further studies for developing more effective combination therapies with amphotericin B and reducing side effects in patients. |
| Databáze: | OpenAIRE |
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