Subsets of cancer cells expressing CX3CR1 are endowed with metastasis-initiating properties and resistance to chemotherapy

Autor: Anthony DiNatale, Ramanpreet Kaur, Chen Qian, Jieyi Zhang, Michael Marchioli, Darin Ipe, Maria Castelli, Chris M. McNair, Gaurav Kumar, Olimpia Meucci, Alessandro Fatatis
Rok vydání: 2022
Předmět:
Zdroj: Oncogene
ISSN: 1476-5594
0950-9232
DOI: 10.1038/s41388-021-02174-w
Popis: Metastasis-initiating cells (MICs) display stem cell-like features, cause metastatic recurrences and defy chemotherapy, which leads to patients’ demise. Here we show that prostate and breast cancer patients harbor contingents of tumor cells with high expression of CX3CR1, OCT4a (POU5F1) and NANOG. Impairing CX3CR1 expression or signaling hampered the formation of tumor spheroids by cell lines from which we isolated small subsets co-expressing CX3CR1 and stemness-related markers, similarly to patients’ tumors. These rare CX3CR1(High) cells show transcriptomic profiles enriched in pathways that regulate pluripotency and are endowed with metastasis-initiating behavior in murine models. Cancer cells lacking these features (CX3CR1(Low)) were capable of re-acquiring CX3CR1-associated features over time, implying that MICs can continuously emerge from non-stem cancer cells. CX3CR1 expression also conferred resistance to docetaxel, and prolonged treatment with docetaxel selected CX3CR1(High) phenotypes with de-enriched transcriptomic profiles for apoptotic pathways. These findings nominate CX3CR1 as novel marker of stem-like tumor cells and provide conceptual ground for future development of approaches targeting CX3CR1 signaling and (re)expression as therapeutic means to prevent or contain metastasis initiation.
Databáze: OpenAIRE
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