Transient expression of a green fluorescent protein in tobacco and maize chloroplast
| Autor: | Edward A. Espinoza-Sánchez, Tania Siqueiros-Cendón, Héctor Lugo-Aguilar, Gerardo Armando Aguado-Santacruz, Quintín Rascón-Cruz, Hugo Varela-Rodríguez, Blanca F. Iglesias-Figueroa, Sigifredo Arévalo-Gallegos |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Inverted repeat lcsh:Biotechnology Plastid transformation Chloroplast transformation Biology 01 natural sciences Applied Microbiology and Biotechnology Genome Marker gene Green fluorescent protein 03 medical and health sciences mgfp5 gene 010608 biotechnology lcsh:TP248.13-248.65 Gene lcsh:QH301-705.5 Genetics Reporter gene Maize Chloroplast Transformation (genetics) 030104 developmental biology lcsh:Biology (General) chloroplast genome Biotechnology |
| Zdroj: | Electronic Journal of Biotechnology, Vol 45, Iss, Pp 1-9 (2020) |
| ISSN: | 0717-3458 |
| Popis: | Background Maize is one of the most important crops worldwide and has been a target of nuclear-based transformation biotechnology to improve it and satisfy the food demand of the ever-growing global population. However, the maize plastid transformation has not been accomplished due to the recalcitrant condition of the crop. Results In this study, we constructed two different vectors with homologous recombination sequences from maize (Zea mays var. LPC13) and grass (Bouteloua gracilis var. ex Steud) (pZmcpGFP and pBgcpGFP, respectively). Both vectors were designed to integrate into rrn23S/rrn16S from an inverted repeat region in the chloroplast genome. Moreover, the vector had the mgfp5 gene driven by Prrn, a leader sequence of the atpB gene and a terminator sequence from the rbcL gene. Also, constructs have an hph gene as a selection marker gene driven by Prrn, a leader sequence from rbcL gene and a terminator sequence from the rbcL gene. Explants of maize, tobacco and Escherichia coli cells were transformed with both vectors to evaluate the transitory expression–an exhibition of green and red fluorescent light under epifluorescence microscopy. These results showed that both vectors were expressed; the reporter gene in all three organisms confirmed the capacity of the vectors to express genes in the cell compartments. Conclusions This paper is the first report of transient expression of GFP in maize embryos and offers new information for genetically improving recalcitrant crops; it also opens new possibilities for the improvement in maize chloroplast transformation with these vectors. How to cite: Arevalo-Gallegos S, Varela-Rodriguez H, Lugo-Aguilar H, et al. Transient expression of a green fluorescent protein in tobacco and maize chloroplast. Electron J Biotechnol 2020;44. https://doi.org/10.1016/j.ejbt.2020.01.008 |
| Databáze: | OpenAIRE |
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