Changes in duodenal CD163-positive cells in dogs with chronic enteropathy after successful treatment

Autor: Albert E. Jergens, Cameron J. Nowell, Lina Maria Martinez Lopez, Wayne G. Kimpton, Caroline S Mansfield, Andrew Stent, Karin Allenspach, Julien R.S. Dandrieux, Simon M. Firestone
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Antigens
CD/metabolism
Macrophages/immunology
Leukocyte L1 Antigen Complex/metabolism
Biomarkers/metabolism
Inflammatory bowel disease
Gastroenterology
Canine
0403 veterinary science
Pathogenesis
Enteropathy
Cell Differentiation
04 agricultural and veterinary sciences
Immunohistochemistry
Infectious Diseases
medicine.anatomical_structure
Antigens
Differentiation
Myelomonocytic/metabolism
Female
medicine.medical_specialty
040301 veterinary sciences
Duodenum
Immunology
Antigens
Differentiation
Myelomonocytic
Receptors
Cell Surface
macrophage
Microbiology
Duodenum/immunology
03 medical and health sciences
Dogs
Antigen
Antigens
CD
inflammatory bowel disease
Internal medicine
Inflammatory Bowel Diseases/drug therapy
medicine
Animals
Humans
Receptors
Cell Surface/metabolism
Molecular Biology
Retrospective Studies
business.industry
Macrophages
Cell Biology
Original Articles
medicine.disease
Inflammatory Bowel Diseases
chronic enteropathy
Disease Models
Animal
030104 developmental biology
CD163
Calprotectin
business
Leukocyte L1 Antigen Complex
Biomarkers
Zdroj: Innate Immunity
Dandrieux, J R, Martinez Lopez, L M, Stent, A, Jergens, A, Allenspach, K, Nowell, C J, Firestone, S M, Kimpton, W & Mansfield, C S 2018, ' Changes in duodenal CD163-positive cells in dogs with chronic enteropathy after successful treatment ', Innate Immunity, vol. 24, no. 7, pp. 400-410 . https://doi.org/10.1177/1753425918799865
ISSN: 1753-4267
DOI: 10.1177/1753425918799865
Popis: Chronic enteropathy (CE) in dogs is characterized retrospectively per treatment response as food-responsive enteropathy (FRE), antibiotic-responsive enteropathy (ARE), and immunosuppressant-responsive enteropathy (IRE) – the latter most resembling inflammatory bowel disease in people. The aim of this study was to characterize duodenal macrophages (Mϕ) in CE using immunohistochemistry; with calprotectin (CAL) as a marker of early differentiated Mϕ and CD163 expression as a marker for resident Mϕ in the duodenum before and after treatment. Prior to treatment, dogs with FRE and IRE had a lower CD163+/CAL+ ratio than control dogs (CTRL) in crypts; this increased significantly and normalized compared with CTRL after treatment. Conversely, the CD163+/CAL+ ratio in dogs with ARE was comparable to that in healthy dogs before and after treatment. In summary, these results suggest that Mϕ play a role in the pathogenesis of CE in FRE and IRE, with a decrease in resident Mϕ and an increase in early differentiated Mϕ, but not in ARE dogs. Mϕ normalize after successful treatment.
Databáze: OpenAIRE
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