Submicroscopic carriage of Plasmodium falciparum and Plasmodium vivax in a low endemic area in Ethiopia where no parasitaemia was detected by microscopy or rapid diagnostic test

Autor: Kjerstin Lanke, Teun Bousema, Helmi Pett, Lynn Grignard, Colin J. Sutherland, Amrish Baidjoe, Chris Drakeley, Fitsum G. Tadesse, Hassen Mamo, Thomas A. Hall
Jazyk: angličtina
Rok vydání: 2015
Předmět:
Male
Plasmodium vivax
Parasitemia
G6PD deficiency
Prevalence
Malaria
Falciparum
Child
GeneralLiterature_REFERENCE(e.g.
dictionaries
encyclopedias
glossaries)
Aged
80 and over
Rapid diagnostic test
Microscopy
biology
Middle Aged
Asymptomatic
Infectious Diseases
Child
Preschool
Carrier State
Female
Inflammatory diseases Radboud Institute for Molecular Life Sciences [Radboudumc 5]
Adult
medicine.medical_specialty
Adolescent
Genotype
Elimination
Plasmodium falciparum
Young Adult
parasitic diseases
medicine
Malaria
Vivax
Humans
Aged
Research
8-aminoquinolines
Infant
Submicroscopic
medicine.disease
biology.organism_classification
Virology
Malaria
Carriage
lnfectious Diseases and Global Health Radboud Institute for Health Sciences [Radboudumc 4]
Cross-Sectional Studies
Parasitology
Tropical medicine
Immunology
Ethiopia
Reagent Kits
Diagnostic
Zdroj: Malaria Journal
Malaria Journal, 14, pp. 303
Malaria Journal, 14, 303
ISSN: 1475-2875
Popis: BACKGROUND: Motivated by the success in malaria control that was documented over the last decade Ethiopia is aiming at malaria elimination by 2020 in selected districts. It is currently unknown if asymptomatic, submicroscopic malaria parasite carriage may form a hurdle to achieve elimination. The elimination effort may further be complicated by possible glucose-6 phosphate dehydrogenase (G6PD) deficiency which would hinder the use of 8-aminoquinolines in the elimination efforts. METHOD: In February 2014 a community-based cross-sectional survey was conducted in Malo, southwest Ethiopia. Finger-prick blood samples (n = 555) were tested for presence of Plasmodium falciparum and Plasmodium vivax with microscopy, rapid diagnostic test (RDT), and nested polymerase chain reaction (nPCR). Multiplicity of P. falciparum infections was determined based on genotyping the polymorphic merozoite surface protein-2 (MSP-2) gene. Individuals were also genotyped for mutations in the gene that produces G6PD. RESULTS: All study participants were malaria infection negative by microscopy and RDT. Nested PCR revealed P. falciparum mono-infection in 5.2% (29/555), P. vivax mono-infection in 4.3% (24/555) and mixed infection in 0.2% (1/555) of individuals. All parasitemic individuals were afebrile (axillary temperature
Databáze: OpenAIRE
Externí odkaz: