Distribution and evaluation of complex carbohydrates in the quail collecting ducts
| Autor: | Daniela Accili, Giovanna Menghi, Maria Gabriella Gabrielli |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Male
Kidney Cortex Tissue Fixation Histology Glycoside Hydrolases Glycoconjugate Renal cortex Population Coturnix Biology Cholangiocyte biology.animal medicine Animals Kidney Tubules Collecting education chemistry.chemical_classification Kidney education.field_of_study Staining and Labeling Histocytochemistry Hydrolysis Metachromasia Mucins Cell Biology General Medicine Quail Staining medicine.anatomical_structure Biochemistry chemistry Carbohydrate Metabolism |
| Zdroj: | Acta Histochemica. 97:43-52 |
| ISSN: | 0065-1281 |
| Popis: | Summary The cell heterogeneity within the collecting duct epithelium of quail kidney was investigated using histochemical methods for complex carbohydrates. The selective distribution of acidic glycoconjugates allowed further discrimination between metachromatic, mucin-secreting cells and dark, proton-transporting cells. Enzymatic digestion by glycosidases was performed to characterize the carboxylated and sulphated glycoconjugates of mucin-secreting cells. The staining intensity of all histochemical reactions with and without prior treatment, was quantitated by means of scanning histophotometry and differences in absorbance values were evaluated by statistical analysis. A different distribution of sulphated components was found for the mucinsecreting cells of renal cortex and medulla, suggesting a morphofunctional heterogeneity within mucin-secreting cell population. |
| Databáze: | OpenAIRE |
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