Ketogenic diet leads to O-GlcNAc modification in the BTBRT+tf/j mouse model of autism
| Autor: | Jane Shearer, Virginia L. Johnsen, Matthias S. Klein, Christopher Newell, Aneal Khan, Warren J. Xu, Jong M. Rho, Nellie C. Yee |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Biochemistry & Molecular Biology Ketogenic medicine.medical_treatment Clinical Sciences Prefrontal Cortex Nerve Tissue Proteins Biology Medical Biochemistry and Metabolomics Transgenic Acetylglucosamine 03 medical and health sciences chemistry.chemical_compound Epilepsy Mice Glucosamine Internal medicine Gene expression medicine Animals Autistic Disorder Autism spectrum disorder Prefrontal cortex Molecular Biology Protein Processing Messenger RNA Animal Post-Translational Metabolism Ketogenic diet medicine.disease Diet carbohydrates (lipids) 030104 developmental biology Endocrinology chemistry Disease Models Molecular Medicine Autism Post-translational modification Biochemistry and Cell Biology Protein O-linked-β-N-acetyl glucosamine |
| Zdroj: | Biochimica et biophysica acta. Molecular basis of disease, vol 1863, iss 9 |
| Popis: | BackgroundProtein O-linked-β-N-acetyl glucosamine (O-GlcNAc) is a post-translational modification to Ser/Thr residues that integrates energy supply with demand. Abnormal O-GlcNAc patterning is evident in several neurological disease states including epilepsy, Alzheimer's disease and autism spectrum disorder (ASD). A potential treatment option for these disorders includes the high-fat, low-carbohydrate, ketogenic diet (KD). The goal of this study was to determine whether the KD induces changes in O-GlcNAc in the BTBRT+tf/j (BTBR) mouse model of ASD.MethodsJuvenile male (5weeks), age-matched C57 or BTBR mice consumed a chow diet (13% kcal fat) or KD (75% kcal fat) for 10-14days. Following these diets, brain (prefrontal cortex) and liver were examined for gene expression levels of key O-GlcNAc mediators, global and protein specific O-GlcNAc as well as indicators of energy status.ResultsThe KD reduced global O-GlcNAc in the livers of all animals (p0.05), yet OGT and OGA expression (mRNA) were elevated in both C57 and BTBR animals (p |
| Databáze: | OpenAIRE |
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