The 14-3-3 cerebrospinal fluid immunoassay lacks utility in the diagnosis of clinical scrapie
| Autor: | Dusty M. Weaver, Kirby D. Smith, Jeff W. Tyler, Jeffrey Lakritz, David C. VanMetre, Allen L. Jenny, Gayle C. Johnson, William D. Taylor |
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| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Tyrosine 3-Monooxygenase 040301 veterinary sciences 030106 microbiology Test sensitivity Scrapie 0403 veterinary science Diagnosis Differential 03 medical and health sciences Cerebrospinal fluid medicine Animals Enzyme Inhibitors False Negative Reactions Immunoassay Sheep General Veterinary medicine.diagnostic_test business.industry 04 agricultural and veterinary sciences 14-3-3 Proteins Immunology Flock business Protein concentration |
| Zdroj: | Scopus-Elsevier |
| Popis: | This study determined whether the immunoassay for cerebrospinal fluid 14-3-3 protein concentration was sensitive and specific in the diagnosis of naturally occurring clinical scrapie in sheep. Cerebrospinal fluid was collected from 9 sheep with the confirmed diagnosis of scrapie. Additionally, cerebrospinal fluid was collected from 13 clinically normal sheep, which originated from a closely monitored flock with no history of scrapie. Sensitivity and specificity were calculated using standard epidemiological methods. Cerebrospinal fluid immunoassay results did not differ significantly between positive and negative sheep. Test sensitivity varied from 0.55 to 0.66, depending on the choice of test endpoint. Test specificity varied from 0.30 to 0.77, depending on the choice of test endpoint. The 14-3-3 cerebrospinal fluid immunoassay appears to have no value in the diagnosis of clinical scrapie in sheep. |
| Databáze: | OpenAIRE |
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