Associations between the Bovine Myostatin Gene and Milk Fatty Acid Composition in New Zealand Holstein-Friesian × Jersey-Cross Cows
| Autor: | Ugonna J. Ekegbu, Huitong Zhou, Jon G.H. Hickford, Ishaku L. Haruna, Hamed Amirpour-Najafabadi, Yunhai Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
myostatin gene Myostatin Article law.invention 03 medical and health sciences Animal science law lcsh:Zoology Genotype lcsh:QL1-991 Polymerase chain reaction Unsaturated fatty acid chemistry.chemical_classification milk lcsh:Veterinary medicine General Veterinary biology 0402 animal and dairy science Intron Nucleic acid sequence Fatty acid food and beverages 04 agricultural and veterinary sciences musculoskeletal system 040201 dairy & animal science 030104 developmental biology chemistry cattle Saturated fatty acid biology.protein lcsh:SF600-1100 Animal Science and Zoology fatty acid variation |
| Zdroj: | Animals : an Open Access Journal from MDPI Animals Volume 10 Issue 9 Animals, Vol 10, Iss 1447, p 1447 (2020) |
| ISSN: | 2076-2615 |
| Popis: | The myostatin gene (MSTN), which encodes the protein myostatin, is pleiotropic, and its expression has been associated with both increased and decreased adipogenesis and increased skeletal muscle mass in animals. In this study, the polymerase chain reaction, coupled with single strand conformation polymorphism analysis, was utilized to reveal nucleotide sequence variation in bovine MSTN in 410 New Zealand (NZ) Holstein-Friesian × Jersey (HF × J)-cross cows. These cows ranged from 3 to 9 years of age and over the time studied, produced an average 22.53 ± 2.18 L of milk per day, with an average milk fat content of 4.94 ± 0.17% and average milk protein content of 4.03 ± 0.10%. Analysis of a 406-bp amplicon from the intron 1 region, revealed five nucleotide sequence variants (A&ndash E) that contained seven nucleotide substitutions. Using general linear mixed-effect model analyses the AD genotype was associated with reduced C10:0, C12:0, and C12:1 levels when compared to levels in cows with the AA genotype. These associations in NZ HF × J cross cows are novel, and they suggest that this variation in bovine MSTN could be explored for increasing the amount of milk unsaturated fatty acid and decreasing the amount of saturated fatty acid. |
| Databáze: | OpenAIRE |
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