Evaluation of carbon isotope fractionation during anaerobic reductive dehalogenation of chlorinated and brominated benzenes

Autor: Seo Yean Sohn, Max M. Häggblom, Ivonne Nijenhuis, Kevin Kuntze
Rok vydání: 2017
Předmět:
0301 basic medicine
Anaerobic
Environmental Engineering
Halogenation
Health
Toxicology and Mutagenesis
030106 microbiology
Trichlorobenzene
Fractionation
010501 environmental sciences
Compound specific carbon isotope analysis
Chemical Fractionation
Chlorobenzenes
01 natural sciences
Gas Chromatography-Mass Spectrometry
Bromobenzenes
Dichlorobenzene
03 medical and health sciences
chemistry.chemical_compound
Isotope fractionation
Rivers
Pentachlorobenzene
medicine
Environmental Chemistry
Organic chemistry
0105 earth and related environmental sciences
Carbon Isotopes
Chemistry
Public Health
Environmental and Occupational Health
Benzene
General Medicine
General Chemistry
Microbial reductive dehalogenation
Pollution
Biodegradation
Environmental
Isotopes of carbon
Environmental chemistry
Halogenated benzenes
Aquatic sediments
medicine.drug
Environmental Monitoring
Zdroj: Chemosphere. 193
ISSN: 1879-1298
Popis: Compound specific stable isotope analysis (CSIA) has been established as a useful tool to evaluate in situ biodegradation. Here, CSIA was used to determine microbial dehalogenation of chloro- and bromobenzenes in microcosms derived from Hackensack River sediments. Gas chromatography-isotope ratio mass spectrometry (GC-IRMS) was used to measure carbon isotope fractionation during reductive dehalogenation of hexachlorobenzene (HCB), pentachlorobenzene (PeCB), 1,2,3,5-tetrachlorobenzene (TeCB), 1,2,3,5-tetrabromobenzene (TeBB), and 1,3,5-tribromobenzene (TriBB). Strong evidence of isotope fractionation coupled to dehalogenation was not observed in the substrate, possibly due to the low solubilities of the highly halogenated benzene substrates and a dilution of the isotope signal. Nonetheless, we could measure a depletion of the ��13C value in the dichlorobenzene product during dechlorination of HCB, the sequential depletion and enrichment of ��13C value for trichlorobenzene in TeCB dechlorinating cultures, and the enrichment of ��13C during debromination of TriBB. This indicates that a measurable isotope fractionation occurred during reductive dehalogenation of highly halogenated chloro- and bromobenzenes in aquatic sediments. Thus, although more quantitative measurements will be needed, the data suggests that CSIA may have application for monitoring in situ microbial reductive dehalogenation of highly halogenated benzenes.
Databáze: OpenAIRE
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