MiR-144 Inhibits Uveal Melanoma Cell Proliferation and Invasion by Regulating c-Met Expression
| Autor: | ZhaoJun Meng, Dejing Shi, Shuyong Mi, Guangfu Dang, Lei Sun, Guangqing Bian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Uveal Neoplasms
C-Met Uveal Neoplasm Down-Regulation Gene Expression lcsh:Medicine Biology chemistry.chemical_compound RNA interference Cell Movement Cell Line Tumor microRNA medicine Humans RNA Messenger lcsh:Science 3' Untranslated Regions Melanoma Cell Proliferation Multidisciplinary Binding Sites Base Sequence Cell growth lcsh:R Transfection Proto-Oncogene Proteins c-met medicine.disease Molecular biology eye diseases Gene Expression Regulation Neoplastic MicroRNAs chemistry Cell culture Cancer research RNA Interference lcsh:Q Research Article |
| Zdroj: | PLoS ONE, Vol 10, Iss 5, p e0124428 (2015) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | MicroRNAs (miRNAs) are a group endogenous small non-coding RNAs that inhibit protein translation through binding to specific target mRNAs. Recent studies have demonstrated that miRNAs are implicated in the development of cancer. However, the role of miR-144 in uveal melanoma metastasis remains largely unknown. MiR-144 was downregulated in both uveal melanoma cells and tissues. Transfection of miR-144 mimic into uveal melanoma cells led to a decrease in cell growth and invasion. After identification of two putative miR-144 binding sites within the 3' UTR of the human c-Met mRNA, miR-144 was proved to inhibit the luciferase activity inMUM-2B cells with a luciferase reporter construct containing the binding sites. In addition, the expression of c-Met protein was inhibited by miR-144. Furthermore, c-Met-mediated cell proliferation and invasion were inhibited by restoration of miR-144 in uveal melanoma cells. In conclusion, miR-144 acts as a tumor suppressor in uveal melanoma, through inhibiting cell proliferation and migration. miR-144 might serve as a potential therapeutic target in uveal melanoma patients. |
| Databáze: | OpenAIRE |
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