Molecular characterization of Oxyspirura mansoni and Philophthalmus gralli collected from the eyes of domestic chickens in Bangladesh
| Autor: | Tadashi Itagaki, Peru Gopal Biswas, Uday Kumar Mohanta, Yuma Ohari |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Genetic Markers
Male 0301 basic medicine Eye Diseases 030231 tropical medicine Zoology Spirurida Infections Trematode Infections Electron Transport Complex IV Oxyspirura mansoni 03 medical and health sciences Monophyly 0302 clinical medicine Philophthalmus gralli parasitic diseases Animals Helminths Clade Poultry Diseases Bangladesh biology Haplotype Helminth Proteins 030108 mycology & parasitology biology.organism_classification Infectious Diseases GenBank Thelazioidea Female Parasitology Trematoda RNA Helminth Chickens |
| Zdroj: | Parasitology International. 80:102243 |
| ISSN: | 1383-5769 |
| DOI: | 10.1016/j.parint.2020.102243 |
| Popis: | A variety of helminths have been found in domestic chickens in Bangladesh, but little is known about their gene sequences. Here, parasitic nematodes and trematodes were collected from the eyes of domestic chickens and analyzed for their morphological and morphometric characteristics, and characterized molecularly. The helminths were identified as Oxyspirura mansoni and Philophthalmus gralli. The ITS1 and ITS2 sequences of O. mansoni were 532 bp and 306 bp in length, respectively, and showed low identity (50.7–62.7%) with those of O. petrowi and O. conjunctivalis. Furthermore, the O. mansoni CO1 sequences (393 bp) showed five haplotypes (97.5–99.5% similarity) that formed a monophyletic clade. With respect to P. gralli, the ITS1 (452 bp) and ITS2 (736 bp) sequences showed 100% similarity with the reference sequences in GenBank. Both the ND1 and CO1 phylograms showed that P. gralli from Bangladesh, Costa Rica and Peru form a monophyletic clade, distinct from the clades of P. lucipetus and P. lacrymosus. Our data show that, Philophthalmus gralli isolates from Bangladesh, Costa Rica and Peru are genetically close to each other. |
| Databáze: | OpenAIRE |
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