Chemical Structure of Bacteriovorax stolpii Lipid A
| Autor: | Michael W. Linscheid, Frederic D. Müller, Eckhard Strauch, Sebastian Beck, Lothar Brecker |
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| Rok vydání: | 2010 |
| Předmět: |
Deltaproteobacteria
Gel electrophoresis chemistry.chemical_classification Chemistry Chemical structure Electrospray ionization Fatty Acids Organic Chemistry Fatty acid Cell Biology Nuclear magnetic resonance spectroscopy Bacteriovorax stolpii Mass spectrometry Biochemistry Lipid A Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Nuclear Magnetic Resonance Biomolecular |
| Zdroj: | Lipids. 45:189-198 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/s11745-010-3383-6 |
| Popis: | Bdellovibrionales is a phylogenetically diverse group of predatory prokaryotes, which consists of the two families Bdellovibrionaceae and Bacteriovoracaceae. We describe LPS and lipid A of the type strain Bacteriovorax stolpii DSM 12778, representing the first characterized endotoxin of a Bacteriovoracaceae member. It has a smooth form LPS, which was identified by SDS-polyacrylamide gel electrophoresis. The lipid A structure was determined by combined gas chromatography-mass spectrometry, electrospray ionization mass spectrometry and NMR spectroscopy. Its backbone consists of two beta-(1--6)-linked 2,3-diamino-2,3-dideoxy-D-glucopyranoses (GlcpN3N) carrying a pyrophosphoethanolamine at O-4' of the non-reducing sugar and a phosphate group linked to O-1 of the reducing GlcpN3N. Positions 2, 3, 2' and 3' of the two GlcpN3N are acylated with primary 3-hydroxy fatty acids and one of those carries a secondary fatty acid. |
| Databáze: | OpenAIRE |
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