Scale-up of aerated bioreactors: CFD validation and application to the enzyme production by Trichoderma reesei
| Autor: | Frederic Augier, Christophe Vial, Vincenzo Cappello, Cecile Plais |
|---|---|
| Přispěvatelé: | IFP Energies nouvelles (IFPEN), Institut Pascal (IP), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
scale-up
General Chemical Engineering Mixing (process engineering) 02 engineering and technology Computational fluid dynamics Multiphase bioreactor non-Newtonian Industrial and Manufacturing Engineering Physics::Fluid Dynamics 020401 chemical engineering Rheology Two-phase model Bioreactor [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology 0204 chemical engineering Trichoderma reesei Complex fluid biology biomass business.industry Applied Mathematics General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification Drag SCALE-UP Environmental science [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph] 0210 nano-technology Biological system business CFD |
| Zdroj: | Chemical Engineering Science Chemical Engineering Science, Elsevier, 2021, 229, pp.116033. ⟨10.1016/j.ces.2020.116033⟩ Chemical Engineering Science, 2021, 229, pp.116033. ⟨10.1016/j.ces.2020.116033⟩ |
| ISSN: | 0009-2509 |
| Popis: | International audience; The application of computational fluid dynamics to aerobic fermentations faces several issues, such as validation of multiphase models at high gas holdup and with complex liquids under turbulent condition. in this work, the eulerian two-fluid model framework was adapted to simulate the enzyme production by the filamentous fungi t. reesei. due to scarcity of data on turbulent mixing in complex fluids, every aspect of the numerical model (turbulence model, drag force law, rheology model, etc.) needed to be validated. first, the adequacy of the model was evaluated by the mean of comparison with new and previous experimental data in non-newtonian aerated systems. once coupled with an apparent oxygen and substrate uptake kinetics, the model was used to investigate the effect of scale-up on the enzyme productivity from biomass. fully predictive results highlighted complex behaviors, such as: possible substrate heterogeneities, yield loss, and non-obvious interactions between mixing and oxygen transfer limitations. |
| Databáze: | OpenAIRE |
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