Microbial fingerprinting detects unique bacterial communities in the faecal microbiota of rats with experimentally-induced colitis
| Autor: | Ashis K. Samanta, Suzanne M. Abimosleh, Nigel J. Percy, Gordon S. Howarth, V. A. Torok |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Gut flora Applied Microbiology and Biotechnology Microbiology Inflammatory bowel disease Rats Sprague-Dawley Pathogenesis Feces medicine Animals Humans Colitis Phylogeny Bacteria biology General Medicine biology.organism_classification medicine.disease DNA Fingerprinting Bacteroidales Rats Gastrointestinal Tract Disease Models Animal Terminal restriction fragment length polymorphism Metagenome Homeostasis |
| Zdroj: | The Journal of Microbiology. 50:218-225 |
| ISSN: | 1976-3794 1225-8873 |
| Popis: | An abnormal composition of the gut microbiota is believed to be associated with the pathogenesis of inflammatory bowel disease (IBD). We utilized terminal restriction fragment length polymorphism (T-RFLP) analysis to quantify faecal bacterial communities from rats with experimental colitis. Male Sprague Dawley rats (n=10/group) ingested 2% dextran sulfate sodium (DSS) or water for up to 7 days. Rats were killed and colonic tissues collected for histological analysis. Damage severity score in the distal colon was significantly greater (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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