Calcium transport in gill plasma membranes of the crab Carcinus Maenas: Evidence for carriers driven by ATP and A Na+ Gradient
| Autor: | P.M. Verbost, Gert Flik, C Lucu, W. Atsma |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Gills
Male Gill animal structures Brachyura Physiology chemistry.chemical_element Aquatic Science Calcium Adenosine Triphosphate medicine Animals Carcinus maenas Molecular Biology Ecology Evolution Behavior and Systematics Calcium metabolism biology Vesicle Calcium-Binding Proteins Cell Membrane Sodium Biological Transport Anatomy biology.organism_classification Epithelium Hypotonic Shock Membrane medicine.anatomical_structure chemistry Insect Science Biophysics Animal Science and Zoology Sodium-Potassium-Exchanging ATPase |
| Zdroj: | Journal of Experimental Biology. 195:109-122 |
| ISSN: | 1477-9145 0022-0949 |
| DOI: | 10.1242/jeb.195.1.109 |
| Popis: | A procedure was developed for the preparation of inside-out vesicles from plasma membranes isolated from the branchial epithelium of the green shore crab Carcinus maenas (L.). Procedures normally applied to fish branchial epithelium required the introduction of an additional hypotonic shock to obtain a preparation containing 22% inside-out vesicles, 33% right-side-out vesicles and 45% leaky membrane fragments. In such membrane preparations, the first direct evidence for uphill (against a [Ca2+] gradient) ATP-dependent and Na+-gradient-dependent Ca2+ transport in crustacean gills was found. The affinity for Ca2+ of the ATP-driven Ca2+ transporter was 149 nmol l−1 and that of the Na+/Ca2+ exchanger was 1.78 μmol l−1; the Vmax values were 1.73 and 9.88 nmol min−1 mg−1 protein respectively. The relative importance of these carriers for Ca2+ transport in the branchial epithelium of the crab is evaluated on the basis of their calcium kinetics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|