Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))
| Autor: | Rommy Díaz, Pablo Contreras, Iván Valdebenito, Kelly Dumorné, Manuel Lee Estevez, Elías Figueroa, Patricio Ulloa Rodríguez, Jennie Risopatrón, Jorge G. Farías |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Osmole lcsh:SH1-691 biology Osmotic concentration Chemistry 010604 marine biology & hydrobiology Motility 04 agricultural and veterinary sciences Genypterus blacodes Aquatic Science biology.organism_classification 01 natural sciences Sperm lcsh:Aquaculture. Fisheries. Angling Animal science Human fertilization Artificial reproduction 040102 fisheries 0401 agriculture forestry and fisheries Animal Science and Zoology Sperm motility |
| Zdroj: | Aquaculture Reports, Vol 11, Iss, Pp 42-46 (2018) |
| ISSN: | 2352-5134 |
| Popis: | In this research we evaluated simple aspects of the sperm biology of Genypterus blacodes, in particular assessing the effects of pH (6, 7, 8 and 9), temperature (4, 8 and 16 °C) and osmolalities 100% sea water (1010 mOsm/kg, Control), 75% sea water (774 mOsm/kg, T1), 50% sea water (488 mOsm/kg, T2) and distilled water (0 mOsm/kg, T3)) on the motility of Genypterus blacodes intratesticular spermatozoa.In addition, we determined the fertilization rate. Our results show that G. blacodes spermatozoa have a sperm density of 5.35 ± 0.16 × 109 spermatozoa/mL. Sperm motility is initiated on contact with a hyperosmotic swimming medium under normal conditions (1010 mOsm/kg, pH 8 and 8 °C). The longest motility duration (432.48 ± 8.89 s) was recorded at 4 °C. The maximum percentage of motile cells was recorded at 8 °C (65.66 ± 4.95) at osmolality 1010 mOsm/kg, whereas an optimum was observed at pH 8. The fertility rate was 73.9 ± 17%. This is the first report on sperm motility of G. blacodes spermatozoa. In conclusion, the results of this study permit a baseline to be established for further research and protocols for artificial reproduction of this species to be developed and optimized. In addition, the information gathered in this research will be useful for developing the biotechnology of Genypterus blacodes. Keywords: Genypterus blacodes, Sperm motility, Osmolality, pH, Temperature |
| Databáze: | OpenAIRE |
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