Wnt5a controls neurite development in olfactory bulb interneurons

Autor: Samuel J. Pleasure, Darya Pino, Youngshik Choe
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Cellular differentiation
Inbred C57BL
CSF
cerebrospinal fluid
Mice
0302 clinical medicine
Neural Stem Cells
non-canonical
2.1 Biological and endogenous factors
Aetiology
10. No inequality
Cells
Cultured
Mice
Knockout
0303 health sciences
Cultured
OB
olfactory bulb
General Neuroscience
Wnt signaling pathway
subventricular zone
subventricular zone (SVZ)
Cell Differentiation
Olfactory Bulb
Neural stem cell
S12
medicine.anatomical_structure
JNK
c-Jun N-terminal kinase
BMP
bone morphogenic protein
Stem Cell Research - Nonembryonic - Non-Human
Research Article
sFRP-1
secreted frizzled-related protein 1
Signal Transduction
Neurite
dendrites
Cells
Knockout
TH
tyrosine hydroxylase
Subventricular zone
SVZ
subventricular zone
Dendrite
Biology
S2
Wnt-5a Protein
lcsh:RC321-571
03 medical and health sciences
Interneurons
medicine
RMS
rostral migratory stream
Neurites
Animals
LRP5/6
LDL (low-density lipoprotein)-receptor-related protein 5/6
NPC
neural progenitor cell
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
030304 developmental biology
LEF
lymphoid enhancer factor
VGAT
vesicular γ-aminobutyric acid transporter
sfrp1
CB
calbindin
Neurosciences
wnt signalling
Stem Cell Research
Olfactory bulb
Mice
Inbred C57BL
Wnt Proteins
DKK1
nervous system
OSN
olfactory sensory axon
TDBTN
total dendritic branch tip number
dkk1
Neurology (clinical)
PFA
paraformaldehyde
Neuroscience
DKK-1
Dickkopf-related protein 1
030217 neurology & neurosurgery
DAPI
4′
6-diamidino-2-phenylindole
Zdroj: ASN NEURO
ASN Neuro, Vol 3 (2011)
ASN Neuro, vol 3, iss 3
ISSN: 1759-0914
Popis: Neurons born in the postnatal SVZ (subventricular zone) must migrate a great distance before becoming mature interneurons of the OB (olfactory bulb). During migration immature OB neurons maintain an immature morphology until they reach their destination. While the morphological development of these cells must be tightly regulated, the cellular pathways responsible are still largely unknown. Our results show that the non-canonical Wnt pathway induced by Wnt5a is important for the morphological development of OB interneurons both in vitro and in vivo. Additionally, we demonstrate that non-canonical Wnt signalling works in opposition to canonical Wnt signalling in neural precursors from the SVZ in vitro. This represents a novel role for Wnt5a in the development of OB interneurons and suggests that canonical and non-canonical Wnt pathways dynamically oppose each other in the regulation of dendrite maturation. © 2011 The Author(s).
Databáze: OpenAIRE
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