Involvement of unconventional myosin VI in myoblast function and myotube formation
| Autor: | Yuriy Rzhepetskyy, Natalia Nowak, Pawel Niewiadomski, Justyna Karolczak, Maria Jolanta Redowicz, Iuliia Pavlyk, Łukasz Majewski, Tomasz J. Prószyński, Elisabeth Ehler, Magdalena Sobczak, Paweł Pomorski, Agata Sikorska |
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| Rok vydání: | 2015 |
| Předmět: |
Talin
Cytoplasm Histology Muscle Fibers Skeletal Neuromuscular junction Golgi Apparatus Biology Endoplasmic Reticulum Muscle Development Cell Line Myoblasts Mice symbols.namesake Cell Movement Myosin Cell Adhesion Animals Myocyte Cell migration Myocytes Cardiac Cell Shape Molecular Biology Actin Cardiomyocytes Original Paper Myosin Heavy Chains Myogenesis Endoplasmic reticulum Actin cytoskeleton Cell Differentiation Cell Biology Vinculin Golgi apparatus musculoskeletal system Rats Cell biology Sarcoplasmic Reticulum Medical Laboratory Technology Differentiation Adhesion biology.protein symbols |
| Zdroj: | Histochemistry and Cell Biology Karolczak, J, Pavlyk, I, Majewski, Ł, Sobczak, M, Niewiadomski, P, Rzhepetskyy, Y, Sikorska, A, Nowak, N, Pomorski, P, Prószyński, T, Ehler, E & Rędowicz, M J 2015, ' Involvement of unconventional myosin VI in myoblast function and myotube formation ', Histochemistry and Cell Biology, vol. 144, no. 1, pp. 21-38 . https://doi.org/10.1007/s00418-015-1322-6 |
| ISSN: | 1432-119X 0948-6143 |
| DOI: | 10.1007/s00418-015-1322-6 |
| Popis: | The important role of unconventional myosin VI (MVI) in skeletal and cardiac muscle has been recently postulated (Karolczak et al. in Histochem Cell Biol 139:873–885, 2013). Here, we addressed for the first time a role for this unique myosin motor in myogenic cells as well as during their differentiation into myotubes. During myoblast differentiation, the isoform expression pattern of MVI and its subcellular localization underwent changes. In undifferentiated myoblasts, MVI-stained puncti were seen throughout the cytoplasm and were in close proximity to actin filaments, Golgi apparatus, vinculin-, and talin-rich focal adhesion as well as endoplasmic reticulum. Colocalization of MVI with endoplasmic reticulum was enhanced during myotube formation, and differentiation-dependent association was also seen in sarcoplasmic reticulum of neonatal rat cardiomyocytes (NRCs). Moreover, we observed enrichment of MVI in myotube regions containing acetylcholine receptor-rich clusters, suggesting its involvement in the organization of the muscle postsynaptic machinery. Overexpression of the H246R MVI mutant (associated with hypertrophic cardiomyopathy) in myoblasts and NRCs caused the formation of abnormally large intracellular vesicles. MVI knockdown caused changes in myoblast morphology and inhibition of their migration. On the subcellular level, MVI-depleted myoblasts exhibited aberrations in the organization of actin cytoskeleton and adhesive structures as well as in integrity of Golgi apparatus and endoplasmic reticulum. Also, MVI depletion or overexpression of H246R mutant caused the formation of significantly wider or aberrant myotubes, respectively, indicative of involvement of MVI in myoblast differentiation. The presented results suggest an important role for MVI in myogenic cells and possibly in myoblast differentiation. |
| Databáze: | OpenAIRE |
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