Transcriptional profiling of identified neurons in leech

Autor: James D. Angstadt, Elizabeth A. C. Heath-Heckman, Veronica B. Lammardo, Maurizio Pellegrino, David A. Weisblat, Christopher J. Winchell, Shinja Yoo, Diana M. Bautista, Francisco F. De-Miguel
Rok vydání: 2021
Předmět:
Central Nervous System
Nervous system
Cell type
lcsh:QH426-470
Bioinformatics
1.1 Normal biological development and functioning
lcsh:Biotechnology
Leech
In situ hybridization
Biology
Medical and Health Sciences
RNASeq
Transcriptome
Transient receptor potential channel
03 medical and health sciences
0302 clinical medicine
Neurobiology
Underpinning research
Hirudo
Information and Computing Sciences
Leeches
lcsh:TP248.13-248.65
Genetics
medicine
2.1 Biological and endogenous factors
Animals
Gene family
Invertebrate
Aetiology
Gene
In Situ Hybridization
030304 developmental biology
Neurons
0303 health sciences
Mechanosensation
Neurosciences
Biological Sciences
biology.organism_classification
Cell biology
lcsh:Genetics
medicine.anatomical_structure
Neurological
Sensory biology
030217 neurology & neurosurgery
Research Article
Biotechnology
Zdroj: BMC Genomics, Vol 22, Iss 1, Pp 1-21 (2021)
BMC Genomics
BMC genomics, vol 22, iss 1
ISSN: 1471-2164
DOI: 10.1186/s12864-021-07526-0
Popis: BackgroundWhile leeches in the genusHirudohave long been models for neurobiology, the molecular underpinnings of nervous system structure and function in this group remain largely unknown. To begin to bridge this gap, we performed RNASeq on pools of identified neurons of the central nervous system (CNS): sensory T (touch), P (pressure) and N (nociception) neurons; neurosecretory Retzius cells; and ganglia from which these four cell types had been removed.ResultsBioinformatic analyses identified 3565 putative genes whose expression differed significantly among the samples. These genes clustered into 9 groups which could be associated with one or more of the identified cell types. We verified predicted expression patterns through in situ hybridization on whole CNS ganglia, and found that orthologous genes were for the most part similarly expressed in a divergent leech genus, suggesting evolutionarily conserved roles for these genes. Transcriptional profiling allowed us to identify candidate phenotype-defining genes from expanded gene families. Thus, we identified one of eight hyperpolarization-activated cyclic-nucleotide gated (HCN) channels as a candidate for mediating the prominent sag current in P neurons, and found that one of five inositol triphosphate receptors (IP3Rs), representing a sub-family of IP3Rs absent from vertebrate genomes, is expressed with high specificity in T cells. We also identified one of twopiezogenes, two of ~ 65 deg/enacgenes, and one of at least 16transient receptor potential(trp) genes as prime candidates for involvement in sensory transduction in the three distinct classes of leech mechanosensory neurons.ConclusionsOur study defines distinct transcriptional profiles for four different neuronal types within the leech CNS, in addition to providing a second ganglionic transcriptome for the species. From these data we identified five gene families that may facilitate the sensory capabilities of these neurons, thus laying the basis for future work leveraging the strengths of the leech system to investigate the molecular processes underlying and linking mechanosensation, cell type specification, and behavior.
Databáze: OpenAIRE
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