Pamidronate Down-regulates Tumor Necrosis Factor-alpha Induced Matrix Metalloproteinases Expression in Human Intervertebral Disc Cells
| Autor: | Jin Oh Park, Byung Ho Lee, Hwan Mo Lee, Hak Sun Kim, Seonghwan Hong, Sangyoon Lee, Jin-Cheol Oh, Young Mi Kang, Seong Hwan Moon, Jae Ho Yang |
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| Rok vydání: | 2016 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine medicine.medical_specialty Endocrinology Diabetes and Metabolism Pamidronate Stimulation Matrix (biology) Matrix metalloproteinase 03 medical and health sciences 0302 clinical medicine Endocrinology Osteoclast Internal medicine medicine Tumor necrosis factor-alpha 030203 arthritis & rheumatology Chemistry Intervertebral disc Metabolism musculoskeletal system In vitro Matrix metalloproteinases 030104 developmental biology medicine.anatomical_structure Original Article Tumor necrosis factor alpha Collagen |
| Zdroj: | Journal of Bone Metabolism |
| ISSN: | 2287-7029 2287-6375 |
| DOI: | 10.11005/jbm.2016.23.3.165 |
| Popis: | Background N-containing bisphosphonates (BPs), such as pamidronate and risedronate, can inhibit osteoclastic function and reduce osteoclast number by inducing apoptotic cell death in osteoclasts. The aim of this study is to demonstrate the effect of pamidronate, second generation nitrogen-containing BPs and to elucidate matrix metallo-proteinases (MMPs) mRNA expression under serum starvation and/or tumor necrosis factor alpha (TNF-α) stimulation on metabolism of intervertebral disc (IVD) cells in vitro. Methods Firstly, to test the effect of pamidronate on IVD cells in vitro, various concentrations (10-12, 10-10, 10-8, and 10-6 M) of pamidronate were administered to IVD cells. Then DNA and proteoglycan synthesis were measured and messenger RNA (mRNA) expressions of type I collagen, type II collagen, and aggrecan were analyzed. Secondly, to elucidate the expression of MMPs mRNA in human IVD cells under the lower serum status, IVD cells were cultivated in full serum or 1% serum. Thirdly, to elucidate the expression of MMPs mRNA in IVD cells under the stimulation of 1% serum and TNF-α (10 ng/mL) In this study, IVD cells were cultivated in three dimensional alginate bead. Results Under the lower serum culture, IVD cells in alginate beads showed upregulation of MMP 2, 3, 9, 13 mRNA. The cells in lower serum and TNF-α also demonstrated upregulation of MMP-2, 3, 9, and 13 mRNA. The cells with various doses of pamidronate and lower serum and TNF-α were reveled partial down-regulation of MMPs. Conclusions Pamidronate, N-containing second generation BPs, was safe in metabolism of IVD in vitro maintaining chondrogenic phenotype and matrix synthesis, and down-regulated TNF-α induced MMPs expression. |
| Databáze: | OpenAIRE |
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