Effect of ethanol supplementation on the transcriptional landscape of bionanocellulose producer Komagataeibacter xylinus E25

Autor:	Stanisław Bielecki, Małgorzata Ryngajłło, Halina Kalinowska, Izabela Cielecka, Paulina Jacek
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Carbohydrate metabolism Komagataeibacter Applied Microbiology and Biotechnology Bacterial cellulose 03 medical and health sciences chemistry.chemical_compound Biosynthesis Gene expression Protein biosynthesis Glycolysis Cellulose Transcriptomics 030304 developmental biology 0303 health sciences Ethanol 030306 microbiology Bionanocellulose Gene Expression Profiling General Medicine Metabolism Gene Expression Regulation Bacterial Culture Media Genomics Transcriptomics Proteomics Glucose chemistry Biochemistry Acetobacteraceae RNA-seq Metabolic Networks and Pathways Biotechnology
Zdroj:	Applied Microbiology and Biotechnology
ISSN:	1432-0614 0175-7598
Popis:	Ethanol exerts a strong positive effect on the cellulose yields from the widely exploited microbial producers of the Komagataeibacter genus. Ethanol is postulated to provide an alternative energy source, enabling effective use of glucose for cellulose biosynthesis rather than for energy acquisition. In this paper, we investigate the effect of ethanol supplementation on the global gene expression profile of Komagataeibacter xylinus E25 using RNA sequencing technology (RNA-seq). We demonstrate that when ethanol is present in the culture medium, glucose metabolism is directed towards cellulose production due to the induction of genes related to UDP-glucose formation and the repression of genes involved in glycolysis and acetan biosynthesis. Transcriptional changes in the pathways of cellulose biosynthesis and c-di-GMP metabolism are also described. The transcript level profiles suggest that Schramm-Hestrin medium supplemented with ethanol promotes bacterial growth by inducing protein biosynthesis and iron uptake. We observed downregulation of genes encoding transposases of the IS110 family which may provide one line of evidence explaining the positive effect of ethanol supplementation on the genotypic stability of K. xylinus E25. The results of this study increase knowledge and understanding of the regulatory effects imposed by ethanol on cellulose biosynthesis, providing new opportunities for directed strain improvement, scaled-up bionanocellulose production, and wider industrial exploitation of the Komagataeibacter species as bacterial cellulose producers. Electronic supplementary material The online version of this article (10.1007/s00253-019-09904-x) contains supplementary material, which is available to authorized users.
Databáze:	OpenAIRE
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